• Target：
• Keap1

• Fields：
• >>Ubiquitin mediated proteolysis;>>Parkinson disease;>>Pathways in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Hepatocellular carcinoma;>>Fluid shear stress and atherosclerosis

• Gene Name：
• KEAP1

• Protein Name：
• Kelch-like ECH-associated protein 1

• Human Gene Id：
• 9817

• Human Swiss Prot No：
• Q14145

• Mouse Gene Id：
• 50868

• Mouse Swiss Prot No：
• Q9Z2X8

• Rat Gene Id：
• 117519

• Rat Swiss Prot No：
• P57790

• Immunogen：
• The antiserum was produced against synthesized peptide derived from the Internal region of human KEAP1. AA range:411-460

• Specificity：
• Keap1 Polyclonal Antibody detects endogenous levels of Keap1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• KEAP1;INRF2;KIAA0132;KLHL19;Kelch-like ECH-associated protein 1;Cytosolic inhibitor of Nrf2;INrf2;Kelch-like protein 19

• Observed Band(KD)：
• 70kD

• Background：
• This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008],

• Function：
• disease:Defects in KEAP1 may be a cause of breast cancer.,disease:Defects in KEAP1 may be involved in non small cell lung carcinomas (NSCLC) and lung adenocarcinoma.,domain:The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.,enzyme regulation:Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane.,function:Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.,PTM:Ubiquitinated and subject to proteasomal degra

• Subcellular Location：
• Cytoplasm . Nucleus . Mainly cytoplasmic (PubMed:15601839). In response to selective autophagy, relocalizes to inclusion bodies following interaction with SQSTM1/p62 (PubMed:20452972). .

• Expression：
• Broadly expressed, with highest levels in skeletal muscle.

Cadmium exposure promotes thyroid pyroptosis and endocrine dysfunction by inhibiting Nrf2/Keap1 signaling ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Yuying Chen, Chengcong Zhou, Yishan Bian, Fangda Fu, Bian'an Zhu, Xuyan Zhao, Muxin Zhang, Chunyuan Zhou, Sai Yao, Zhiguo Zhang, Huan Luo, Yuying Ge, Chengliang Wu, Hongfeng Ruan IF Mouse thyroid

The Keap1/Nrf2-ARE signaling pathway is involved in atrazine induced dopaminergic neurons degeneration via microglia activation. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2021 Dec;226:112862 WB Rat 1:1000 Neuron BV-2 cell

Modulations of Keap1-Nrf2 signaling axis by TIIA ameliorated the oxidative stress-induced myocardial apoptosis. FREE RADICAL BIOLOGY AND MEDICINE Free Radical Bio Med. 2018 Feb;115:191 WB,IF Mouse Myocardial Neonatal rat ventricular myocytes (NRVMs)

Active Peptide KF-8 from Rice Bran Attenuates Oxidative Stress in a Mouse Model of Aging Induced by d-Galactose. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY J Agr Food Chem. 2020;68(44):12271–12283 WB Mouse 1 : 1000 Liver NIH/3T3 cell

Curcumin protects against methylmercury-induced cytotoxicity in primary rat astrocytes by activating the Nrf2/ARE pathway independently of PKCδ. TOXICOLOGY Toxicology. 2019 Sep;425:152248 WB Rat astrocytes

Paeonol protects against acute pancreatitis by Nrf2 and NF-κB pathways in mice JOURNAL OF PHARMACY AND PHARMACOLOGY Короткова Ирина Павловна WB Mouse

Cryptotanshinone alleviates lipopolysaccharide and cigarette smoke-induced chronic obstructive pulmonary disease in mice via the Keap1/Nrf2 axis. Jianwen Chen WB Human BEAS-2B cell

AR/PCC herb pair inhibits osteoblast pyroptosis to alleviate diabetes-related osteoporosis by activating Nrf2/Keap1 pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Hongfeng Ruan IF Rat 1:500 vertebral body

Ginsenoside Rg1 attenuates lipopolysaccharide-induced chronic liver damage by activating Nrf2 signaling and inhibiting inflammasomes in hepatic cells JOURNAL OF ETHNOPHARMACOLOGY Huimin Zhou WB Mouse 1:1000 liver tissue

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