• Target：
• Keap1

• Fields：
• >>Ubiquitin mediated proteolysis;>>Parkinson disease;>>Pathways in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Hepatocellular carcinoma;>>Fluid shear stress and atherosclerosis

• Gene Name：
• KEAP1

• Protein Name：
• Kelch-like ECH-associated protein 1

• Human Gene Id：
• 9817

• Human Swiss Prot No：
• Q14145

• Mouse Gene Id：
• 50868

• Mouse Swiss Prot No：
• Q9Z2X8

• Rat Gene Id：
• 117519

• Rat Swiss Prot No：
• P57790

• Immunogen：
• The antiserum was produced against synthesized peptide derived from the Internal region of human KEAP1. AA range:411-460

• Specificity：
• Keap1 Polyclonal Antibody detects endogenous levels of Keap1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• KEAP1;INRF2;KIAA0132;KLHL19;Kelch-like ECH-associated protein 1;Cytosolic inhibitor of Nrf2;INrf2;Kelch-like protein 19

• Observed Band(KD)：
• 70kD

• Background：
• This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008],

• Function：
• disease:Defects in KEAP1 may be a cause of breast cancer.,disease:Defects in KEAP1 may be involved in non small cell lung carcinomas (NSCLC) and lung adenocarcinoma.,domain:The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.,enzyme regulation:Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane.,function:Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.,PTM:Ubiquitinated and subject to proteasomal degra

• Subcellular Location：
• Cytoplasm . Nucleus . Mainly cytoplasmic (PubMed:15601839). In response to selective autophagy, relocalizes to inclusion bodies following interaction with SQSTM1/p62 (PubMed:20452972). .

• Expression：
• Broadly expressed, with highest levels in skeletal muscle.

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