• Target：
• IL-1β

• Fields：
• >>Antifolate resistance;>>MAPK signaling pathway;>>Cytokine-cytokine receptor interaction;>>NF-kappa B signaling pathway;>>Necroptosis;>>Osteoclast differentiation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>Cytosolic DNA-sensing pathway;>>C-type lectin receptor signaling pathway;>>Hematopoietic cell lineage;>>IL-17 signaling pathway;>>Th17 cell differentiation;>>TNF signaling pathway;>>Inflammatory mediator regulation of TRP channels;>>Non-alcoholic fatty liver disease;>>AGE-RAGE signaling pathway in diabetic complications;>>Alcoholic liver disease;>>Type I diabetes mellitus;>>Alzheimer disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Pertussis;>>Legionellosis;>>Yersinia infection;>>Leishmaniasis;>>Chagas disease;>>African trypanosomiasis;>>Malaria;>>Amoebiasis;>>Tuberculosis;>>Measles;>>Human cytomegalovirus infection;>>Influenza A;>>Herpes simp

• Gene Name：
• IL1B

• Protein Name：
• Interleukin-1 beta

• Human Gene Id：
• 3553

• Human Swiss Prot No：
• P01584

• Mouse Gene Id：
• 16176

• Mouse Swiss Prot No：
• P10749

• Rat Swiss Prot No：
• Q63264

• Immunogen：
• The antiserum was produced against synthesized peptide derived from the Internal region of human IL1B. AA range:181-230

• Specificity：
• IL-1β Polyclonal Antibody detects endogenous levels of IL-1β protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• IL1B;IL1F2;Interleukin-1 beta;IL-1 beta;Catabolin

• Observed Band(KD)：
• 17kD

• Background：
• The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008],

• Function：
• domain:The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.,function:Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.,online information:Interleukin-1 entry,online information:The Singapore human mutation and polymorphism database,similarity:Belongs to the IL-1 family.,subcellular location:The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.,subunit:Mono

• Subcellular Location：
• Cytoplasm, cytosol . Secreted . Lysosome . Secreted, extracellular exosome . The precursor is cytosolic (PubMed:15192144). In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted (PubMed:24201029, PubMed:33377178, PubMed:33883744). Mature form is secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33883744). In contrast, the precursor form is not released, due to the presence of an acidic region that is proteolytically removed by CASP1 during maturation (PubMed:33883744). The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10 (PubMed:32272059). .

• Expression：
• Expressed in activated monocytes/macrophages (at protein level).

Loss of ATG5 in KRT14+ cells leads to accumulated functional impairments of salivary glands via pyroptosis

Dietary Fatty Acid Regulation of the NLRP3 Inflammasome via the TLR4/NF-κB Signaling Pathway Affects Chondrocyte Pyroptosis WB,IHC Mouse 1 : 100,1 : 500 articular cartilage/

AIP1 suppresses neovascularization by inhibiting the NOX4-induced NLRP3/NLRP6 imbalance in a murine corneal alkali burn model WB Mouse 1:200 corneas/

Salidroside attenuates high altitude hypobaric hypoxia-induced brain injury in mice via inhibiting NF-κB/NLRP3 pathway WB Mouse 1:1000 hippocampus/

Effect of Radix Polygalae extract on the colonic dysfunction in rats induced by chronic restraint stress WB Rat 1:1000 colon /

Effect of Radix Polygalae extract on the colonic dysfunction in rats induced by chronic restraint stress WB Rat 1:1000 colon /

Polydatin Improves Sepsis-Associated Encephalopathy by Activating Sirt1 and Reducing p38 Phosphorylation. JOURNAL OF SURGICAL RESEARCH2022 Aug;276:379. Mouse brain tissue

LncRNA TUG1 Promotes Apoptosis, Invasion, and Angiogenesis of Retinal Endothelial Cells in Retinopathy of Prematurity via MiR-145-5p. Frontiers in Medicine2022 Apr 4;9:803214. Mouse 1:200 retinal tissue

Crocin alleviates cognitive impairment associated with atherosclerosis via improving neuroinflammation in LDLR−/− mice fed a high-fat/cholesterol diet 2022 Jan 27. WB Mouse 1:1000 hippocampus,parietal cortex

Mechanical loading mitigates osteoarthritis symptoms by regulating the inflammatory microenvironment in a mouse model 2022 Feb 27. WB,IHC Mouse 1:200 articular cartilage

The Long-Noncoding RNA TUG1 Regulates Oxygen-Induced Retinal Neovascularization in Mice via MiR-299 Invest Ophth Vis Sci. 2022 Jan;63(1):37-37. WB,IF,IHC Mouse 1:1000,1:200 retina

Huang-Lian-Jie-Du decoction attenuates cognitive dysfunction of rats with type 2 diabetes by regulating autophagy and NLRP3 inflammasome activation J Ethnopharmacol. 2022 Jun;292:115196. WB Rat Hippocampus

Attenuation of Oxidative Stress-Induced Cell Apoptosis and Pyroptosis in RSC96 Cells by Salvianolic Acid B Chin J Integr Med. 2022 Jan;:1-6. WB Rat 1:800

Oat fiber attenuates circulating oxysterols levels and hepatic inflammation via targeting TLR4 signal pathway in LDL receptor knockout mice. Journal of Functional Foods J Funct Foods. 2021 Jan;76:104322 WB Mouse Liver

Elevated c-fos expression is correlated with phenotypic switching of human vascular smooth muscle cells derived from lower limb venous varicosities. Journal of Vascular Surgery-Venous and Lymphatic Disorders J Vasc Surg-Venous L. 2021 Jan;9:242 WB Human 1:1000 VSMCs

Maternally expressed gene 3 regulates retinal neovascularization in retinopathy of prematurity. Neural Regeneration Research Neural Regen Res. 2022 Jun; 17(6): 1364–1368 WB Mouse 1:1000 Retina

Flavonoid-Rich Ethanol Extract from the Leaves of Diospyros kaki Attenuates D-Galactose-Induced Oxidative Stress and Neuroinflammation-Mediated Brain Aging in Mice. Oxidative Medicine and Cellular Longevity 2018 Dec 23 WB Mouse 1 : 1000 cortex,hippocampus

Amelioration of Neuropathic Pain and Attenuation of Neuroinflammation Responses by Tetrahydropalmatine Through the p38MAPK/NF-κB/iNOS Signaling Pathways in Animal and Cellular Models. INFLAMMATION Inflammation. 2021 Nov;:1-13 WB Mouse 1:500 Dorsal root ganglion (DRG)，sciatic nerve (SN) N2a cells ，BV2 microglia cell

Cereal Fiber Ameliorates High-Fat/Cholesterol-Diet-Induced Atherosclerosis by Modulating the NLRP3 Inflammasome Pathway in ApoE–/– Mice. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY J Agr Food Chem. 2018;66(19):4827–4834 WB Mouse 1:1000 aortas

Flavonoid-rich ethanol extract from the leaves of Diospyros kaki attenuates cognitive deficits, amyloid-beta production, oxidative stress, and neuroinflammation in APP/PS1 transgenic mice. BRAIN RESEARCH Brain Res. 2018 Jan;1678:85 WB Mouse 1:1000 Brain

Metadherin silencing results in the inhibition of pyroptosis in lipopolysaccharide/adenosine triphosphate − stimulated renal tubular epithelial cells. TISSUE & CELL Tissue Cell. 2022 Apr;75:101722 WB Rat 1:1000 NRK-52E cell

Low-dose alcohol ameliorated homocysteine-induced anxiety-related behavior via attenuating oxidative stress in mice. NEUROSCIENCE LETTERS Neurosci Lett. 2020 Jan;714:134568 WB Mouse 1:2000 hippocampal tissue

MCC950 Inhibits NLRP3 Inflammasome and Alleviates Axonal Injures in Early Stages of Diffuse Axonal Injury in Rats. NEUROCHEMICAL RESEARCH Neurochem Res. 2020 Sep;45(9):2020-2031 WB Rat 1:1000 Cortex

Small Peptides Compound Isolated from Agkistrodon with Antiarthritic Effect in Collagen-Induced Arthritis Rats. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2018;2018:8265150 WB Rat hind limb

Jiang, Kang-feng, et al. "Polydatin ameliorates Staphylococcus aureus-induced mastitis in mice via inhibiting TLR2-mediated activation of the p38 MAPK/NF-κB pathway." Acta Pharmacologica Sinica 38.2 (2017): 211.

Lv, Xiaopei, et al. "TIIA attenuates LPS-induced mouse endometritis by suppressing the NF-κB signaling pathway." Canadian journal of physiology and pharmacology 93.11 (2015): 967-971.

Zhang, Wen, et al. "Parathyroid hormone-related peptide (1–34) reduces alveolar bone loss in type 1 diabetic rats." Archives of oral biology 83 (2017): 13-19.

Monti, Daria M. "Role of Antioxidants in the Protection from Aging-Related Diseases."

HHcy Induces Pyroptosis and Atherosclerosis via the Lipid Raft-Mediated NOX-ROS-NLRP3 Inflammasome Pathway in apoE−/− Mice Cells Hongmei Tan WB Mouse,Human

Identification of CCR2 as a hub in septic myocardial injury and cardioprotection of silibinin FREE RADICAL BIOLOGY AND MEDICINE Ye Tian WB Mouse cardiomyocytes

TMBIM4 Deficiency Facilitates NLRP3 Inflammasome Activation-Induced Pyroptosis of Trophoblasts: A Potential Pathogenesis of Preeclampsia Biology-Basel Chunyan Liu WB Human placental tissues HTR-8/SVneo cells

A novel gene therapy for methamphetamine- induced cognitive disorder with a hyper-acidified fusion variant of DnaJB1 Molecular Therapy-Nucleic Acids Zhurong Zou WB Mouse hippocampus BV2 cell

Tryptophan-kynurenine metabolic characterization in the gut and brain of depressive-like rats induced by chronic restraint stress JOURNAL OF AFFECTIVE DISORDERS Rui-Le Pan WB Rat hippocampus,colon

Tryptophan-kynurenine metabolic characterization in the gut and brain of depressive-like rats induced by chronic restraint stress JOURNAL OF AFFECTIVE DISORDERS Rui-Le Pan WB Rat hippocampus,colon

Puerarin protects against sepsis-associated encephalopathy by inhibiting NLRP3/Caspase-1/GSDMD pyroptosis pathway and reducing blood-brain barrier damage EUROPEAN JOURNAL OF PHARMACOLOGY Min Zhao WB Mouse,Rat brain tissues Murine hippocampal neurons (HT22)

Synergistic effects of combined treatment of 1,2-dichloroethane and high-dose ethanol on liver damage in mice and the related mechanisms. Yaping Jin WB Mouse 1:1000 liver

Apple polyphenol extract alleviates DSS-induced ulcerative colitis and linked behavioral disorders via regulating the gut-brain axis. Xinli Li WB Mouse 1:1000 colon tissue

Targeting the mechanism of IRF3 in sepsis-associated acute kidney injury via the Hippo pathway. Xingui Dai WB Human HK-2 cell

Pristimerin suppresses AIM2 inflammasome by modulating AIM2-PYCARD/ASC stability via selective autophagy to alleviate tendinopathy. Autophagy Xiao Yu WB，IF Mouse Achilles tendon Peritoneal macrophages

A novel drug combination of Tofacitinib and Iguratimod alleviates rheumatoid arthritis and secondary osteoporosis. INTERNATIONAL IMMUNOPHARMACOLOGY Qiang Shu IHC Rat 1:100 Synovial tissue,bone tissue

Production of recombinant human long-acting IL-18 binding protein: inhibitory effect on ulcerative colitis in mice. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY Xiao Yechen WB Mouse colonic tissues

AAV2-antiVEGFscFv gene therapy for retinal neovascularization. Molecular Therapy-Methods & Clinical Development Guangzuo Luo IHC Mouse eyes

Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4+ T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity LIFE SCIENCES Lu Yu WB Rat 1:1000 lymphocytes

Increased RBP4 and Asprosin Are Novel Contributors in Inflammation Process of Periodontitis in Obese Rats INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Yuwei Zhang WB Rat 1:2000

Nobiletin Alleviated Epithelial–Mesenchymal Transition of Hepatocytes in Liver Fibrosis Based on Autophagy—Hippo/YAP Pathway MOLECULAR NUTRITION & FOOD RESEARCH Yuting Hao WB Mouse,Human liver tissue L02 cell

Cucurbitacin E reduces IL-1β-induced inflammation and cartilage degeneration by inhibiting the PI3K/Akt pathway in osteoarthritic chondrocytes Journal of Translational Medicine Wang Lin WB,IHC Mouse,Human 1:1000,1:100 articular cartilage Chondrocytes

Sympathetic Neurotransmitter, VIP, Delays Intervertebral Disc Degeneration via FGF18/FGFR2-Mediated Activation of Akt Signaling Pathway Advanced Biology Kaiqiang Sun WB Human nucleus pulposus (NP) cell

Low-carbohydrate high-fat dietary pattern ameliorates alcohol-related liver disease via a 6PGD-involved mechanism in mice Food & Function Songtao Li WB Mouse liver tissue

Huang-Lian-Jie-Du decoction drug-containing serum inhibits IL-1β secretion from D-glucose and PA induced BV2 cells via autophagy/NLRP3 signaling JOURNAL OF ETHNOPHARMACOLOGY Ruimin Tian WB Mouse 1:1000 BV2 cell

Cathepsin B aggravates atherosclerosis in ApoE-deficient mice by modulating vascular smooth muscle cell pyroptosis through NF-κB / NLRP3 signaling pathway PLoS One Hui Li WB Mouse vascular smooth muscle cells (VSMCs)

Association of GAL-8 promoter methylation levels with coronary plaque inflammation INTERNATIONAL JOURNAL OF CARDIOLOGY Bing Xia IHC Human 1:100 coronary tissue

Apple polyphenol extract ameliorates sugary-diet-induced depression-like behaviors in male C57BL/6 mice by inhibiting inflammation of the gut-brain axis Food & Function Yisha Xie WB Mouse 1:1000 colon tissue,hippocampus tissue

The recovery of intestinal barrier function and changes in oral microbiota after radiation therapy injury Frontiers in Cellular and Infection Microbiology Kun Wang WB Human 1:4000 intestinal mucosal tissue

The relationship of ALPK1, hyaluronic acid and M1 macrophage polarization in the temporomandibular joint synovitis JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Jie Zhao IHC,WB Mouse 1:400,1:1000 temporomandibular joint synovium RAW264.7 cell

The Scutellaria-Coptis herb couple and its active small-molecule ingredient wogonoside alleviate cytokine storm by regulating the CD39/NLRP3/GSDMD signaling pathway JOURNAL OF ETHNOPHARMACOLOGY Huan Wang WB Human lung tissue THP-1 cell,RAW264.7 cell

Exploring the effect of Clostridium butyricum on lung injury associated with acute pancreatitis in mice by combined 16S rRNA and metabolomics analysis ANAEROBE Jiaxin Liu IHC Mouse 1:400 pancreatic tissue,lung tissue

MiR-21-5p modulates LPS-induced acute injury in alveolar epithelial cells by targeting SLC16A10 Scientific Reports Zeng Huanan WB Human 1:1000 A549 cell

Puerariae lobatae Radix ameliorates chronic kidney disease by reshaping gut microbiota and downregulating Wnt/β‑catenin signaling Molecular Medicine Reports Peng Wu WB,IHC Mouse 1:1000 kidney tissue,colon tissue

Clostridium butyricum upregulates GPR109A/AMPK/PGC-1α and ameliorates acute pancreatitis-associated intestinal barrier injury in mice ARCHIVES OF MICROBIOLOGY Deng Guiqing WB,IHC Mouse 1:400 pancreas tissue, small intestine tissue, colon tissue

Ameliorated Effects of Fucoidan on Dextran Sulfate Sodium-Induced Ulcerative Colitis and Accompanying Anxiety and Depressive Behaviors in Aged C57BL/6 Mice JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY Shilan Li WB Mouse 1:1000 colon tissue

Ethyl pyruvate alleviates NLRP3/Caspase-1/GSDMD-mediated neuronal pyroptosis in neonatal rats with hypoxic–ischemic brain damage INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE Sha Sha WB Rat,Mouse 1:1000 hippocampal tissue HT22 cell

Mitochondria-targeted drug delivery system based on tetrahedral framework nucleic acids for bone regeneration under oxidative stress CHEMICAL ENGINEERING JOURNAL Yanjing Li WB Rat Primary periodontal stem cell(PDLSC)

Gut microbiota promotes macrophage M1 polarization in hepatic sinusoidal obstruction syndrome via regulating intestinal barrier function mediated by butyrate Gut Microbes Si Zhao WB Mouse 1:1000 liver tissue

Chromodomain Y‐like (CDYL) inhibition ameliorates acute kidney injury in mice by regulating tubular pyroptosis ACTA PHARMACOLOGICA SINICA Xiang Ting IHC,IF Human 1:100 kidney tissue

Macrophage WNK1 senses intracellular hypo-chlorine to regulate vulnerability to sepsis attack during hypochloremia INTERNATIONAL IMMUNOPHARMACOLOGY Tianyue Yin WB Mouse bone marrow-derived macrophages (BMDMs),Raw264.7 cell

Gastrodin against oxidative stress-inflammation crosstalk via inhibiting mtDNA/TLR9 and JAK2/STAT3 signaling to ameliorate ischemic stroke injury INTERNATIONAL IMMUNOPHARMACOLOGY Menglian Zhang WB Rat 1:2000 brain tissue

Up-regulation of IL-1β and sPLA2-III in the medial prefrontal cortex contributes to orofacial and somatic hyperalgesia induced by malocclusion via glial-neuron crosstalk EUROPEAN JOURNAL OF PHARMACOLOGY Hai-Nan Feng WB,IF Rat 1:1000,1:100 brain tissue

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