• Target：
• IL-1β

• Fields：
• >>Antifolate resistance;>>MAPK signaling pathway;>>Cytokine-cytokine receptor interaction;>>NF-kappa B signaling pathway;>>Necroptosis;>>Osteoclast differentiation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>Cytosolic DNA-sensing pathway;>>C-type lectin receptor signaling pathway;>>Hematopoietic cell lineage;>>IL-17 signaling pathway;>>Th17 cell differentiation;>>TNF signaling pathway;>>Inflammatory mediator regulation of TRP channels;>>Non-alcoholic fatty liver disease;>>AGE-RAGE signaling pathway in diabetic complications;>>Alcoholic liver disease;>>Type I diabetes mellitus;>>Alzheimer disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Pertussis;>>Legionellosis;>>Yersinia infection;>>Leishmaniasis;>>Chagas disease;>>African trypanosomiasis;>>Malaria;>>Amoebiasis;>>Tuberculosis;>>Measles;>>Human cytomegalovirus infection;>>Influenza A;>>Herpes simp

• Gene Name：
• IL1B

• Protein Name：
• Interleukin-1 beta

• Human Gene Id：
• 3553

• Human Swiss Prot No：
• P01584

• Mouse Gene Id：
• 16176

• Mouse Swiss Prot No：
• P10749

• Rat Swiss Prot No：
• Q63264

• Immunogen：
• The antiserum was produced against synthesized peptide derived from the Internal region of human IL1B. AA range:181-230

• Specificity：
• IL-1β Polyclonal Antibody detects endogenous levels of IL-1β protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• IL1B;IL1F2;Interleukin-1 beta;IL-1 beta;Catabolin

• Observed Band(KD)：
• 17kD

• Background：
• The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008],

• Function：
• domain:The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.,function:Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.,online information:Interleukin-1 entry,online information:The Singapore human mutation and polymorphism database,similarity:Belongs to the IL-1 family.,subcellular location:The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.,subunit:Mono

• Subcellular Location：
• Cytoplasm, cytosol . Secreted . Lysosome . Secreted, extracellular exosome . The precursor is cytosolic (PubMed:15192144). In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted (PubMed:24201029, PubMed:33377178, PubMed:33883744). Mature form is secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33883744). In contrast, the precursor form is not released, due to the presence of an acidic region that is proteolytically removed by CASP1 during maturation (PubMed:33883744). The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10 (PubMed:32272059). .

• Expression：
• Expressed in activated monocytes/macrophages (at protein level).

Loss of ATG5 in KRT14+ cells leads to accumulated functional impairments of salivary glands via pyroptosis

Dietary Fatty Acid Regulation of the NLRP3 Inflammasome via the TLR4/NF-κB Signaling Pathway Affects Chondrocyte Pyroptosis WB,IHC Mouse 1 : 100,1 : 500 articular cartilage/

AIP1 suppresses neovascularization by inhibiting the NOX4-induced NLRP3/NLRP6 imbalance in a murine corneal alkali burn model WB Mouse 1:200 corneas/

Salidroside attenuates high altitude hypobaric hypoxia-induced brain injury in mice via inhibiting NF-κB/NLRP3 pathway WB Mouse 1:1000 hippocampus/

Effect of Radix Polygalae extract on the colonic dysfunction in rats induced by chronic restraint stress WB Rat 1:1000 colon /

Effect of Radix Polygalae extract on the colonic dysfunction in rats induced by chronic restraint stress WB Rat 1:1000 colon /

Polydatin Improves Sepsis-Associated Encephalopathy by Activating Sirt1 and Reducing p38 Phosphorylation. JOURNAL OF SURGICAL RESEARCH2022 Aug;276:379. Mouse brain tissue

LncRNA TUG1 Promotes Apoptosis, Invasion, and Angiogenesis of Retinal Endothelial Cells in Retinopathy of Prematurity via MiR-145-5p. Frontiers in Medicine2022 Apr 4;9:803214. Mouse 1:200 retinal tissue

Crocin alleviates cognitive impairment associated with atherosclerosis via improving neuroinflammation in LDLR−/− mice fed a high-fat/cholesterol diet 2022 Jan 27. WB Mouse 1:1000 hippocampus,parietal cortex

Mechanical loading mitigates osteoarthritis symptoms by regulating the inflammatory microenvironment in a mouse model 2022 Feb 27. WB,IHC Mouse 1:200 articular cartilage

The Long-Noncoding RNA TUG1 Regulates Oxygen-Induced Retinal Neovascularization in Mice via MiR-299 Invest Ophth Vis Sci. 2022 Jan;63(1):37-37. WB,IF,IHC Mouse 1:1000,1:200 retina

Huang-Lian-Jie-Du decoction attenuates cognitive dysfunction of rats with type 2 diabetes by regulating autophagy and NLRP3 inflammasome activation J Ethnopharmacol. 2022 Jun;292:115196. WB Rat Hippocampus

Attenuation of Oxidative Stress-Induced Cell Apoptosis and Pyroptosis in RSC96 Cells by Salvianolic Acid B Chin J Integr Med. 2022 Jan;:1-6. WB Rat 1:800

Oat fiber attenuates circulating oxysterols levels and hepatic inflammation via targeting TLR4 signal pathway in LDL receptor knockout mice. Journal of Functional Foods J Funct Foods. 2021 Jan;76:104322 WB Mouse Liver

Elevated c-fos expression is correlated with phenotypic switching of human vascular smooth muscle cells derived from lower limb venous varicosities. Journal of Vascular Surgery-Venous and Lymphatic Disorders J Vasc Surg-Venous L. 2021 Jan;9:242 WB Human 1:1000 VSMCs

Maternally expressed gene 3 regulates retinal neovascularization in retinopathy of prematurity. Neural Regeneration Research Neural Regen Res. 2022 Jun; 17(6): 1364–1368 WB Mouse 1:1000 Retina

Flavonoid-Rich Ethanol Extract from the Leaves of Diospyros kaki Attenuates D-Galactose-Induced Oxidative Stress and Neuroinflammation-Mediated Brain Aging in Mice. Oxidative Medicine and Cellular Longevity 2018 Dec 23 WB Mouse 1 : 1000 cortex,hippocampus

Amelioration of Neuropathic Pain and Attenuation of Neuroinflammation Responses by Tetrahydropalmatine Through the p38MAPK/NF-κB/iNOS Signaling Pathways in Animal and Cellular Models. INFLAMMATION Inflammation. 2021 Nov;:1-13 WB Mouse 1:500 Dorsal root ganglion (DRG)，sciatic nerve (SN) N2a cells ，BV2 microglia cell

Cereal Fiber Ameliorates High-Fat/Cholesterol-Diet-Induced Atherosclerosis by Modulating the NLRP3 Inflammasome Pathway in ApoE–/– Mice. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY J Agr Food Chem. 2018;66(19):4827–4834 WB Mouse 1:1000 aortas

Flavonoid-rich ethanol extract from the leaves of Diospyros kaki attenuates cognitive deficits, amyloid-beta production, oxidative stress, and neuroinflammation in APP/PS1 transgenic mice. BRAIN RESEARCH Brain Res. 2018 Jan;1678:85 WB Mouse 1:1000 Brain

Metadherin silencing results in the inhibition of pyroptosis in lipopolysaccharide/adenosine triphosphate − stimulated renal tubular epithelial cells. TISSUE & CELL Tissue Cell. 2022 Apr;75:101722 WB Rat 1:1000 NRK-52E cell

Low-dose alcohol ameliorated homocysteine-induced anxiety-related behavior via attenuating oxidative stress in mice. NEUROSCIENCE LETTERS Neurosci Lett. 2020 Jan;714:134568 WB Mouse 1:2000 hippocampal tissue

MCC950 Inhibits NLRP3 Inflammasome and Alleviates Axonal Injures in Early Stages of Diffuse Axonal Injury in Rats. NEUROCHEMICAL RESEARCH Neurochem Res. 2020 Sep;45(9):2020-2031 WB Rat 1:1000 Cortex

Small Peptides Compound Isolated from Agkistrodon with Antiarthritic Effect in Collagen-Induced Arthritis Rats. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2018;2018:8265150 WB Rat hind limb

Jiang, Kang-feng, et al. "Polydatin ameliorates Staphylococcus aureus-induced mastitis in mice via inhibiting TLR2-mediated activation of the p38 MAPK/NF-κB pathway." Acta Pharmacologica Sinica 38.2 (2017): 211.

Lv, Xiaopei, et al. "TIIA attenuates LPS-induced mouse endometritis by suppressing the NF-κB signaling pathway." Canadian journal of physiology and pharmacology 93.11 (2015): 967-971.

Zhang, Wen, et al. "Parathyroid hormone-related peptide (1–34) reduces alveolar bone loss in type 1 diabetic rats." Archives of oral biology 83 (2017): 13-19.

Monti, Daria M. "Role of Antioxidants in the Protection from Aging-Related Diseases."

HHcy Induces Pyroptosis and Atherosclerosis via the Lipid Raft-Mediated NOX-ROS-NLRP3 Inflammasome Pathway in apoE−/− Mice Cells Hongmei Tan WB Mouse,Human

Identification of CCR2 as a hub in septic myocardial injury and cardioprotection of silibinin FREE RADICAL BIOLOGY AND MEDICINE Ye Tian WB Mouse cardiomyocytes

TMBIM4 Deficiency Facilitates NLRP3 Inflammasome Activation-Induced Pyroptosis of Trophoblasts: A Potential Pathogenesis of Preeclampsia Biology-Basel Chunyan Liu WB Human placental tissues HTR-8/SVneo cells

A novel gene therapy for methamphetamine- induced cognitive disorder with a hyper-acidified fusion variant of DnaJB1 Molecular Therapy-Nucleic Acids Zhurong Zou WB Mouse hippocampus BV2 cell

Tryptophan-kynurenine metabolic characterization in the gut and brain of depressive-like rats induced by chronic restraint stress JOURNAL OF AFFECTIVE DISORDERS Rui-Le Pan WB Rat hippocampus,colon

Tryptophan-kynurenine metabolic characterization in the gut and brain of depressive-like rats induced by chronic restraint stress JOURNAL OF AFFECTIVE DISORDERS Rui-Le Pan WB Rat hippocampus,colon

Puerarin protects against sepsis-associated encephalopathy by inhibiting NLRP3/Caspase-1/GSDMD pyroptosis pathway and reducing blood-brain barrier damage EUROPEAN JOURNAL OF PHARMACOLOGY Min Zhao WB Mouse,Rat brain tissues Murine hippocampal neurons (HT22)

Synergistic effects of combined treatment of 1,2-dichloroethane and high-dose ethanol on liver damage in mice and the related mechanisms. Yaping Jin WB Mouse 1:1000 liver

Apple polyphenol extract alleviates DSS-induced ulcerative colitis and linked behavioral disorders via regulating the gut-brain axis. Xinli Li WB Mouse 1:1000 colon tissue

Targeting the mechanism of IRF3 in sepsis-associated acute kidney injury via the Hippo pathway. Xingui Dai WB Human HK-2 cell

Pristimerin suppresses AIM2 inflammasome by modulating AIM2-PYCARD/ASC stability via selective autophagy to alleviate tendinopathy. Autophagy Xiao Yu WB，IF Mouse Achilles tendon Peritoneal macrophages

A novel drug combination of Tofacitinib and Iguratimod alleviates rheumatoid arthritis and secondary osteoporosis. INTERNATIONAL IMMUNOPHARMACOLOGY Qiang Shu IHC Rat 1:100 Synovial tissue,bone tissue

Production of recombinant human long-acting IL-18 binding protein: inhibitory effect on ulcerative colitis in mice. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY Xiao Yechen WB Mouse colonic tissues

AAV2-antiVEGFscFv gene therapy for retinal neovascularization. Molecular Therapy-Methods & Clinical Development Guangzuo Luo IHC Mouse eyes

Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4+ T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity LIFE SCIENCES Lu Yu WB Rat 1:1000 lymphocytes

Increased RBP4 and Asprosin Are Novel Contributors in Inflammation Process of Periodontitis in Obese Rats INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Yuwei Zhang WB Rat 1:2000

Nobiletin Alleviated Epithelial–Mesenchymal Transition of Hepatocytes in Liver Fibrosis Based on Autophagy—Hippo/YAP Pathway MOLECULAR NUTRITION & FOOD RESEARCH Yuting Hao WB Mouse,Human liver tissue L02 cell

Cucurbitacin E reduces IL-1β-induced inflammation and cartilage degeneration by inhibiting the PI3K/Akt pathway in osteoarthritic chondrocytes Journal of Translational Medicine Wang Lin WB,IHC Mouse,Human 1:1000,1:100 articular cartilage Chondrocytes

Sympathetic Neurotransmitter, VIP, Delays Intervertebral Disc Degeneration via FGF18/FGFR2-Mediated Activation of Akt Signaling Pathway Advanced Biology Kaiqiang Sun WB Human nucleus pulposus (NP) cell

Low-carbohydrate high-fat dietary pattern ameliorates alcohol-related liver disease via a 6PGD-involved mechanism in mice Food & Function Songtao Li WB Mouse liver tissue

Huang-Lian-Jie-Du decoction drug-containing serum inhibits IL-1β secretion from D-glucose and PA induced BV2 cells via autophagy/NLRP3 signaling JOURNAL OF ETHNOPHARMACOLOGY Ruimin Tian WB Mouse 1:1000 BV2 cell

Cathepsin B aggravates atherosclerosis in ApoE-deficient mice by modulating vascular smooth muscle cell pyroptosis through NF-κB / NLRP3 signaling pathway PLoS One Hui Li WB Mouse vascular smooth muscle cells (VSMCs)

Association of GAL-8 promoter methylation levels with coronary plaque inflammation INTERNATIONAL JOURNAL OF CARDIOLOGY Bing Xia IHC Human 1:100 coronary tissue

Apple polyphenol extract ameliorates sugary-diet-induced depression-like behaviors in male C57BL/6 mice by inhibiting inflammation of the gut-brain axis Food & Function Yisha Xie WB Mouse 1:1000 colon tissue,hippocampus tissue

The recovery of intestinal barrier function and changes in oral microbiota after radiation therapy injury Frontiers in Cellular and Infection Microbiology Kun Wang WB Human 1:4000 intestinal mucosal tissue

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