• Target：
• BNIP-3

• Fields：
• >>FoxO signaling pathway;>>Mitophagy - animal;>>Autophagy - animal;>>Shigellosis;>>Legionellosis

• Gene Name：
• BNIP3

• Protein Name：
• BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

• Human Gene Id：
• 664

• Human Swiss Prot No：
• Q12983

• Mouse Swiss Prot No：
• O55003

• Immunogen：
• The antiserum was produced against synthesized peptide derived from the Internal region of human BNIP3. AA range:71-120

• Specificity：
• BNIP-3 Polyclonal Antibody detects endogenous levels of BNIP-3 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• BNIP3;NIP3;BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

• Observed Band(KD)：
• 22kD

• Background：
• This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014],

• Function：
• function:Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.,similarity:Belongs to the NIP3 family.,subcellular location:Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope.,subunit:Homodimer. Binds to BCL2. Interacts with BNIP3L. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1.,

• Subcellular Location：
• Mitochondrion. Mitochondrion outer membrane; Single-pass membrane protein. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane.

• Expression：
• B-cell,Brain,Cerebellum,Kidney,Testis,

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Quantitative Proteomic Analysis Reveals That Arctigenin Alleviates Concanavalin A-Induced Hepatitis Through Suppressing Immune System and Regulating Autophagy. Frontiers in Immunology 2018 Aug 16 WB Mouse liver

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