• Target：
• IL-8

• Fields：
• >>Cytokine-cytokine receptor interaction;>>Viral protein interaction with cytokine and cytokine receptor;>>Chemokine signaling pathway;>>NF-kappa B signaling pathway;>>Phospholipase D signaling pathway;>>Cellular senescence;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>IL-17 signaling pathway;>>Non-alcoholic fatty liver disease;>>AGE-RAGE signaling pathway in diabetic complications;>>Alcoholic liver disease;>>Epithelial cell signaling in Helicobacter pylori infection;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Pertussis;>>Legionellosis;>>Yersinia infection;>>Chagas disease;>>Malaria;>>Amoebiasis;>>Hepatitis B;>>Human cytomegalovirus infection;>>Influenza A;>>Kaposi sarcoma-associated herpesvirus infection;>>Coronavirus disease - COVID-19;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Bladder cancer;>>Rheumatoid arthritis;>>Lipid and atherosclerosis

• Gene Name：
• IL8 CXCL8

• Protein Name：
• Interleukin-8

• Human Gene Id：
• 3576

• Human Swiss Prot No：
• P10145

• Immunogen：
• The antiserum was produced against synthesized peptide derived from the C-terminal region of human IL8. AA range:50-99

• Specificity：
• IL-8 Polyclonal Antibody detects endogenous levels of IL-8 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• IL8;CXCL8;Interleukin-8;IL-8;C-X-C motif chemokine 8;Emoctakin;Granulocyte chemotactic protein 1;GCP-1;Monocyte-derived neutrophil chemotactic factor;MDNCF;Monocyte-derived neutrophil-activating peptide;MONAP;Neutrophil-activating protein 1;NAP-1;Protein 3-10C;T-cell chemotactic factor

• Observed Band(KD)：
• 11kD

• Background：
• The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008],

• Function：
• function:IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.,online information:Interleukin-8 entry,PTM:Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.,similarity:Belongs to the intercrine alpha (chemokine CxC) family.,subunit:Homodimer.,

• Subcellular Location：
• Secreted.

• Expression：
• Chronic myeloid leukemia cell,Kidney,Lung,Lung carcinoma,Neutrophil,Periphe

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Association between higher expression of interleukin-8 (IL-8) and haplotype −353A/−251A/+678T of IL-8 gene with preeclampsia: A case–control study. MEDICINE Medicine. 2016 Dec; 95(52): e5537 IHC Human 1:200 Placental tissue

CXCL5 as an autocrine or paracrine cytokine is associated with proliferation and migration of hepatoblastoma HepG2 cells. Oncology Letters Oncol Lett. 2017 Dec;14(6):7977-7985 WB Human 1:1000 HepG2 cells

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Li, Na, et al. "Asparaginyl endopeptidase may promote liver sinusoidal endothelial cell angiogenesis via PI3K/Akt pathway." Revista espanola de enfermedades digestivas: organo oficial de la Sociedad Espanola de Patologia Digestiva111 (2018).

Helicobacter pylori with trx1 high expression promotes gastric diseases via upregulating the IL23A/NF-κB/IL8 pathway HELICOBACTER Xin Guan IHC Human 1:200 antrum tissue

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