DNA pol ε B Polyclonal Antibody
- Catalog No.：YT5065
- Applications：WB;ELISA
- Reactivity：Human;Rat;Mouse;
- Target：
- DNA pol ε B
- Fields：
- >>DNA replication;>>Base excision repair;>>Nucleotide excision repair
- Gene Name：
- POLE2
- Protein Name：
- DNA polymerase epsilon subunit 2
- Human Gene Id：
- 5427
- Human Swiss Prot No：
- P56282
- Mouse Swiss Prot No：
- O54956
- Immunogen：
- Synthesized peptide derived from the Internal region of human DNA pol ε B.
- Specificity：
- DNA pol ε B Polyclonal Antibody detects endogenous levels of DNA pol ε B protein.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Polyclonal, Rabbit,IgG
- Dilution：
- WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- POLE2;DPE2;DNA polymerase epsilon subunit 2;DNA polymerase II subunit 2;DNA polymerase epsilon subunit B
- Observed Band(KD)：
- 59kD
- Background：
- catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,function:Participates in DNA repair and in chromosomal DNA replication.,miscellaneous:In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.,similarity:Belongs to the DNA polymerase epsilon subunit B family.,subunit:Consists of four subunits: POLE, POLE2, POLE3 and POLE4.,
- Function：
- catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,function:Participates in DNA repair and in chromosomal DNA replication.,miscellaneous:In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.,similarity:Belongs to the DNA polymerase epsilon subunit B family.,subunit:Consists of four subunits: POLE, POLE2, POLE3 and POLE4.,
- Subcellular Location：
- Nucleus.
- Expression：
- T cells,
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- Antibody-FAQs
- Products Images
- Western Blot analysis of extracts from Jurkat cells, using DNA pol ε B Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
