• Target：
• V-ATPase D1

• Fields：
• >>Oxidative phosphorylation;>>Metabolic pathways;>>Lysosome;>>Phagosome;>>Synaptic vesicle cycle;>>Collecting duct acid secretion;>>Vibrio cholerae infection;>>Epithelial cell signaling in Helicobacter pylori infection;>>Tuberculosis;>>Human papillomavirus infection;>>Viral carcinogenesis;>>Rheumatoid arthritis

• Gene Name：
• ATP6V0D1

• Protein Name：
• V-type proton ATPase subunit d 1

• Human Gene Id：
• 9114

• Human Swiss Prot No：
• P61421

• Mouse Gene Id：
• 11972

• Mouse Swiss Prot No：
• P51863

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human V-ATPase D1. AA range:221-270

• Specificity：
• V-ATPase D1 Polyclonal Antibody detects endogenous levels of V-ATPase D1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ATP6V0D1;ATP6D;VPATPD;V-type proton ATPase subunit d 1;V-ATPase subunit d 1;32 kDa accessory protein;V-ATPase 40 kDa accessory protein;V-ATPase AC39 subunit;p39;Vacuolar proton pump subunit d 1

• Observed Band(KD)：
• 40kD

• Background：
• This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously. [pro

• Function：
• function:Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis.,similarity:Belongs to the V-ATPase V0D/AC39 subunit family.,subunit:V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d).,tissue specificity:Ubiquitous.,

• Subcellular Location：
• Membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome membrane ; Peripheral membrane protein . Cytoplasmic vesicle, clathrin-coated vesicle membrane ; Peripheral membrane protein . Localizes to centrosome and the base of the cilium. .

• Expression：
• Ubiquitous.

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