ACK Polyclonal Antibody

    • Catalog No.:YT5018
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human,Mouse
      • Gene Name:
      • TNK2
      • Protein Name:
      • Activated CDC42 kinase 1
      • Human Swiss Prot No:
      • Q07912
      • Mouse Swiss Prot No:
      • O54967
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human ACK1. AA range:250-299
      • Specificity:
      • ACK Polyclonal Antibody detects endogenous levels of ACK protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • TNK2; ACK1; Activated CDC42 kinase 1; ACK-1; Tyrosine kinase non-receptor protein 2
      • MolecularWeight(Da):
      • 114327
      • Observed Band(KD):
      • 115
      • Background:
      • tyrosine kinase non receptor 2(TNK2) Homo sapiens This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Magnesium.,enzyme regulation:The SH3 domain appears to play an autoinhibitory role.,function:Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development.,sequence caution:Unlikely isoform. Aberrant splice sites.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Contains 1 CRIB domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH3 domain.,subunit:Interacts with CDC42. Interacts with activated CSPG4.,
      • Subcellular Location:
      • nucleus,cytoplasm,endosome,plasma membrane,clathrin-coated pit,adherens junction,membrane,clathrin-coated vesicle,cytoplasmic vesicle membrane,extrinsic component of cytoplasmic side of plasma membrane,Grb2-EGFR complex,
      • Expression:
      • Brain,Epithelium,Hippocampus,Thalamus,Uterus,
      • Products Images
      • Western Blot analysis of A549 cells using ACK Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Western blot analysis of the lysates from COLO205 cells using ACK1 antibody.