• Target：
• β-1,4-Gal-T1

• Fields：
• >>Galactose metabolism;>>N-Glycan biosynthesis;>>Various types of N-glycan biosynthesis;>>Other types of O-glycan biosynthesis;>>Mannose type O-glycan biosynthesis;>>Glycosaminoglycan biosynthesis - keratan sulfate;>>Glycosphingolipid biosynthesis - lacto and neolacto series;>>Metabolic pathways

• Gene Name：
• B4GALT1

• Protein Name：
• Beta-1,4-galactosyltransferase 1

• Human Gene Id：
• 2683

• Human Swiss Prot No：
• P15291

• Mouse Gene Id：
• 14595

• Mouse Swiss Prot No：
• P15535

• Immunogen：
• Synthesized peptide derived from the C-terminal region of human β-1,4-Gal-T1.

• Specificity：
• β-1,4-Gal-T1 Polyclonal Antibody detects endogenous levels of β-1,4-Gal-T1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• B4GALT1;GGTB2;Beta-1;4-galactosyltransferase 1;Beta-1,4-GalTase 1;Beta4Gal-T1;b4Gal-T1;UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1;UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1

• Observed Band(KD)：
• 50kD

• Background：
• This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lacto

• Function：
• catalytic activity:UDP-galactose + D-glucose = UDP + lactose.,catalytic activity:UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.,catalytic activity:UDP-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.,cofactor:Manganese.,disease:Defects in B4GALT1 are the cause of congenital disorder of glycosylation type 2D (CDG2D) [MIM:607091]. CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins duri

• Subcellular Location：
• [Isoform Long]: Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein. Cell membrane ; Single-pass type II membrane protein. Cell surface . Cell projection, filopodium . Found in trans cisternae of Golgi but is mainly localized at the plasma membrane (PubMed:1714903). B4GALT1 cell surface expression is regulated by UBE2Q1 (By similarity). .; [Isoform Short]: Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein. Found in trans cisternae of Golgi. .; [Processed beta-1,4-galactosyltransferase 1]: Secreted . Soluble form found in body fluids. .

• Expression：
• Ubiquitously expressed, but at very low levels in fetal and adult brain.

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