• Target：
• WAVE2

• Fields：
• >>Adherens junction;>>Fc gamma R-mediated phagocytosis;>>Regulation of actin cytoskeleton;>>Bacterial invasion of epithelial cells;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Yersinia infection;>>Choline metabolism in cancer

• Gene Name：
• WASF2

• Protein Name：
• Wiskott-Aldrich syndrome protein family member 2

• Human Gene Id：
• 10163

• Human Swiss Prot No：
• Q9Y6W5

• Mouse Gene Id：
• 242687

• Mouse Swiss Prot No：
• Q8BH43

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human WASF2. AA range:141-190

• Specificity：
• WAVE2 Polyclonal Antibody detects endogenous levels of WAVE2 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• WASF2;WAVE2;Wiskott-Aldrich syndrome protein family member 2;WASP family protein member 2;Protein WAVE-2;Verprolin homology domain-containing protein 2

• Observed Band(KD)：
• 55kD

• Background：
• This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. The published map location (PMID:10381382) has been changed based on recent genomic sequence comparisons, which indicate that the expressed gene is located on chromosome 1, and a pseudogene may be located on chromosome X. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011],

• Function：
• domain:Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.,function:Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton.,similarity:Belongs to the SCAR/WAVE family.,similarity:Contains 1 WH2 domain.,subcellular location:At the interface between the lamellipodial actin meshwork and the membrane.,subunit:Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. Directly interacts with C3orf10/HSPC300.,tissue specificity:Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle.,

• Subcellular Location：
• Cytoplasm, cytoskeleton . Cell projection, lamellipodium . Basolateral cell membrane . At the interface between the lamellipodial actin meshwork and the membrane. .

• Expression：
• Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle.

Semen Ziziphi Spinosae attenuates blood–brain barrier dysfunction induced by lipopolysaccharide by targeting the FAK-DOCK180-Rac1-WAVE2-Arp3 signaling pathway WB Rat,Human brain/hCMECs/D3 cells

Dragon’s Blood Regulates Rac1-WAVE2-Arp2/3 Signaling Pathway to Protect Rat Intestinal Epithelial Barrier Dysfunction Induced by Simulated Microgravity. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Int J Mol Sci. 2021 Jan;22(5):2722 WB Rat 1:1000 Ileum

Cucurbitacin B inhibits the migration and invasion of breast cancer cells by altering the biomechanical properties of cells. PHYTOTHERAPY RESEARCH Phytother Res. 2019 Mar;33(3):618-630 WB Human 1:1000 MDA-MB-231 cell， SKBR-3 cell

WAVE2 Enhanced Hepatic Stellate Cells Activity in Colorectal Liver Metastases. Cancer Management and Research Cancer Manag Res. 2020; 12: 7671–7680 IHC,WB Human 1:500 HSCs cell

Mangiferin inhibits chronic stress-induced tumor growth in colorectal liver metastases via WAVE2 signaling pathway Heliyon Lei Pei WB Mouse,Human Hepatic stellate cells (HSCs) ,HT29 cell

Dragon’s blood attenuates LPS-induced intestinal epithelial barrier dysfunction via upregulation of FAK-DOCK180-Rac1-WAVE2-Arp3 and downregulation of TLR4/NF-κB signaling pathways Journal of Natural Medicines Liu Huayan WB Rat 1:2000 ileum mucosa tissue

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