WAVE2 Polyclonal Antibody

    • Catalog No.:YT4898
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • WASF2
      • Protein Name:
      • Wiskott-Aldrich syndrome protein family member 2
      • Human Swiss Prot No:
      • Q9Y6W5
      • Mouse Swiss Prot No:
      • Q8BH43
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human WASF2. AA range:141-190
      • Specificity:
      • WAVE2 Polyclonal Antibody detects endogenous levels of WAVE2 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • WASF2; WAVE2; Wiskott-Aldrich syndrome protein family member 2; WASP family protein member 2; Protein WAVE-2; Verprolin homology domain-containing protein 2
      • MolecularWeight(Da):
      • 54284
      • Observed Band(KD):
      • 55
      • Background:
      • WAS protein family member 2(WASF2) Homo sapiens This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. The published map location (PMID:10381382) has been changed based on recent genomic sequence comparisons, which indicate that the expressed gene is located on chromosome 1, and a pseudogene may be located on chromosome X. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011],
      • Function:
      • domain:Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.,function:Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton.,similarity:Belongs to the SCAR/WAVE family.,similarity:Contains 1 WH2 domain.,subcellular location:At the interface between the lamellipodial actin meshwork and the membrane.,subunit:Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. Directly interacts with C3orf10/HSPC300.,tissue specificity:Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle.,
      • Subcellular Location:
      • ruffle,intracellular,early endosome,cytosol,cytoskeleton,cell-cell adherens junction,actin cytoskeleton,lamellipodium,SCAR complex,extracellular exosome,
      • Expression:
      • Epithelium,Lymph,Placenta,T-cell lymphoma,Uterus,
      • Products Images
      • Western Blot analysis of 293 cells using WAVE2 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Western blot analysis of WASF2 Antibody. The lane on the right is blocked with the WASF2 peptide.
      • Immunohistochemistryt analysis of paraffin-embedded human pancreas, using WASF2 Antibody. The lane on the right is blocked with the WASF2 peptide.
      • Western blot analysis of the lysates from K562 cells using WASF2 antibody.