• Target：
• USP16

• Gene Name：
• USP16

• Protein Name：
• Ubiquitin carboxyl-terminal hydrolase 16

• Human Gene Id：
• 10600

• Human Swiss Prot No：
• Q9Y5T5

• Mouse Gene Id：
• 74112

• Mouse Swiss Prot No：
• Q99LG0

• Rat Gene Id：
• 288306

• Rat Swiss Prot No：
• Q2KJ09

• Immunogen：
• Synthesized peptide derived from the Internal region of human USP16.

• Specificity：
• USP16 Polyclonal Antibody detects endogenous levels of USP16 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• USP16;MSTP039;Ubiquitin carboxyl-terminal hydrolase 16;Deubiquitinating enzyme 16;Ubiquitin thioesterase 16;Ubiquitin-processing protease UBP-M;Ubiquitin-specific-processing protease 16

• Observed Band(KD)：
• 93kD

• Background：
• This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:Ubiquitin C-terminal thioester + H(2)O = ubiquitin + a thiol.,disease:A chromosomal aberration involving USP16 is a cause of Chronic myelomonocytic leukemia. Inversion inv(21) (q21;q22) with RUNX1/AML1.,domain:The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin.,function:Specifically deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-10' of histone H3, and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.,PTM:Ph

• Subcellular Location：
• Nucleus .

• Expression：
• Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

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