UBE3C Polyclonal Antibody
- Catalog No.：YT4802
- Applications：WB;ELISA
- Reactivity：Human;Rat;Mouse;
- Target：
- UBE3C
- Fields：
- >>Ubiquitin mediated proteolysis
- Gene Name：
- UBE3C
- Protein Name：
- Ubiquitin-protein ligase E3C
- Human Gene Id：
- 148581
- Human Swiss Prot No：
- Q15386
- Mouse Swiss Prot No：
- Q80U95
- Immunogen：
- Synthesized peptide derived from the Internal region of human UBE3C.
- Specificity：
- UBE3C Polyclonal Antibody detects endogenous levels of UBE3C protein.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Polyclonal, Rabbit,IgG
- Dilution：
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- UBE3C;KIAA0010;KIAA10;Ubiquitin-protein ligase E3C;HectH2
- Observed Band(KD)：
- 125kD
- Background：
- function:E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.,miscellaneous:A cysteine residue is required for ubiquitin-thioester formation.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.,similarity:Contains 1 IQ domain.,subunit:Interacts with 26S proteasomes. Binds CAND2.,tissue specificity:Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas.,
- Function：
- function:E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.,miscellaneous:A cysteine residue is required for ubiquitin-thioester formation.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.,similarity:Contains 1 IQ domain.,subunit:Interacts with 26S proteasomes. Binds CAND2.,tissue specificity:Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas.,
- Subcellular Location：
- proteasome complex,intracellular,nucleus,cytoplasm,
- Expression：
- Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas.
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using UBE3C Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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- Western Blot analysis of HuvEc cells using UBE3C Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
