• Target：
• UBE3B

• Fields：
• >>Ubiquitin mediated proteolysis

• Gene Name：
• UBE3B

• Protein Name：
• Ubiquitin-protein ligase E3B

• Human Gene Id：
• 89910

• Human Swiss Prot No：
• Q7Z3V4

• Mouse Swiss Prot No：
• Q9ES34

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human UBE3B. AA range:581-630

• Specificity：
• UBE3B Polyclonal Antibody detects endogenous levels of UBE3B protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• UBE3B;Ubiquitin-protein ligase E3B

• Observed Band(KD)：
• 123kD

• Background：
• The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [pro

• Function：
• function:E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.,similarity:Contains 1 IQ domain.,tissue specificity:Widely expressed.,

• Subcellular Location：
• nucleus,cytoplasm,

• Expression：
• Widely expressed.

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