TLK2 Polyclonal Antibody

    • Catalog No.:YT4674
    • Applications:WB,ELISA
    • Reactivity:Human,Mouse
      • Gene Name:
      • TLK2
      • Protein Name:
      • Serine/threonine-protein kinase tousled-like 2
      • Human Swiss Prot No:
      • Q86UE8
      • Mouse Swiss Prot No:
      • O55047
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human TLK2. AA range:191-240
      • Specificity:
      • TLK2 Polyclonal Antibody detects endogenous levels of TLK2 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • TLK2; Serine/threonine-protein kinase tousled-like 2; HsHPK; PKU-alpha; Tousled-like kinase 2
      • MolecularWeight(Da):
      • 87661
      • Observed Band(KD):
      • 90
      • Background:
      • tousled like kinase 2(TLK2) Homo sapiens This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013],
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Cell-cycle regulated, maximal activity in S-phase. Inactivated by phosphorylation at Ser-750, potentially by CHK1.,function:Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Heterodimerizes with TLK1. Interacts with ASF1A and ASF1B.,tissue specificity:Widely expressed. Present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. Also found in adult cell lines.,
      • Subcellular Location:
      • nucleus,intermediate filament,cell junction,perinuclear region of cytoplasm,
      • Expression:
      • Epithelium,Placenta,Testis,
      • Products Images
      • Western Blot analysis of various cells using TLK2 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Western blot analysis of lysates from COLO cells, using TLK2 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from HeLa cells using TLK2 antibody.