• Target：
• TIF1α

• Gene Name：
• TRIM24

• Protein Name：
• Transcription intermediary factor 1-alpha

• Human Gene Id：
• 8805

• Human Swiss Prot No：
• O15164

• Mouse Gene Id：
• 21848

• Mouse Swiss Prot No：
• Q64127

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human TRIM24. AA range:1001-1050

• Specificity：
• TIF1α Polyclonal Antibody detects endogenous levels of TIF1α protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• TRIM24;RNF82;TIF1;TIF1A;Transcription intermediary factor 1-alpha;TIF1-alpha;E3 ubiquitin-protein ligase TRIM24;RING finger protein 82;Tripartite motif-containing protein 24

• Observed Band(KD)：
• 117kD

• Background：
• The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008],

• Function：
• disease:A chromosomal aberration involving TIF1 is a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Translocation t(7;10)(q32;q11) with RET. The translocation generates the TIF1/RET (PTC6) oncogene.,function:Interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors requires the presence of estradiol.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 bromo domain.,similarity:Contains 1 PHD-type zinc finger.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 2 B box-type zinc fingers.,subunit:Interacts with CBX1 and CBX3 (By similarity). Interacts with NR3C2.,

• Subcellular Location：
• Nucleus . Cytoplasm . Mitochondrion . Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear. Translocated from nucleus to mitochondria to mediate antiviral immunity (PubMed:32324863). .

• Expression：
• Brain,Epithelium,Mammary cancer,Testis,Thyroid,

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