• Target：
• TdT

• Fields：
• >>Non-homologous end-joining;>>Hematopoietic cell lineage

• Gene Name：
• DNTT

• Protein Name：
• DNA nucleotidylexotransferase

• Human Gene Id：
• 1791

• Human Swiss Prot No：
• P04053

• Mouse Swiss Prot No：
• P09838

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human DNTT. AA range:381-430

• Specificity：
• TdT Polyclonal Antibody detects endogenous levels of TdT protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IHC: 100-300.WB 1:500 - 1:2000. ELISA: 1:40000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• DNTT;TDT;DNA nucleotidylexotransferase;Terminal addition enzyme;Terminal deoxynucleotidyltransferase;Terminal transferase

• Observed Band(KD)：
• 60kD

• Background：
• This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Magnesium.,disease:Very high levels of enzyme activity have been detected in certain acute leukemic cells.,function:Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.,similarity:Belongs to the DNA polymerase type-X family.,similarity:Contains 1 BRCT domain.,subunit:Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA.,

• Subcellular Location：
• Nucleus .

• Expression：
• Testis,Thymus,

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