• Target：
• SR-4

• Fields：
• >>Calcium signaling pathway;>>cAMP signaling pathway;>>Neuroactive ligand-receptor interaction;>>Serotonergic synapse

• Gene Name：
• HTR4

• Protein Name：
• 5-hydroxytryptamine receptor 4

• Human Gene Id：
• 3360

• Human Swiss Prot No：
• Q13639

• Mouse Gene Id：
• 15562

• Mouse Swiss Prot No：
• P97288

• Rat Gene Id：
• 25324

• Rat Swiss Prot No：
• Q62758

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human 5-HT-4. AA range:21-70

• Specificity：
• SR-4 Polyclonal Antibody detects endogenous levels of SR-4 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• HTR4;5-hydroxytryptamine receptor 4;5-HT-4;5-HT4;Serotonin receptor 4

• Observed Band(KD)：
• 43kD

• Background：
• This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010],

• Function：
• alternative products:Additional isoforms seem to exist,function:This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.,similarity:Belongs to the G-protein coupled receptor 1 family.,subcellular location:Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.,subunit:Isoform 5-HT4(A) interacts with MAGI2, MPP3, SLC9A3R1 and SNX27 isoforms 1 and 2. Isoform 5-HT4(E) interacts with INADL, NOS1 and SEC23A. Isoform 5-HT4(A) forms a complex including SLC9A3R1 and EZR.,tissue specificity:Isoform 5-HT4(A) is expressed in ileum, brain, and atrium, but not in the ventricle.

• Subcellular Location：
• Cell membrane; Multi-pass membrane protein. Endosome. Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli. .

• Expression：
• Isoform 5-HT4(A) is expressed in ileum, brain, and atrium, but not in the ventricle.

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