• Target：
• Siah-1/2

• Fields：
• >>p53 signaling pathway;>>Ubiquitin mediated proteolysis;>>Wnt signaling pathway

• Gene Name：
• SIAH1/SIAH2

• Protein Name：
• E3 ubiquitin-protein ligase SIAH1/2

• Human Gene Id：
• 6477/6478

• Human Swiss Prot No：
• Q8IUQ4/O43255

• Mouse Gene Id：
• 20439

• Rat Gene Id：
• 140941/140593

• Rat Swiss Prot No：
• Q920M9/Q8R4T2

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human SIAH1. AA range:181-230

• Specificity：
• Siah-1/2 Polyclonal Antibody detects endogenous levels of Siah-1/2 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• SIAH1;HUMSIAH;E3 ubiquitin-protein ligase SIAH1;Seven in absentia homolog 1;Siah-1;Siah-1a;SIAH2;E3 ubiquitin-protein ligase SIAH2;Seven in absentia homolog 2;Siah-2;hSiah2

• Observed Band(KD)：
• 34kD

• Background：
• This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008],

• Function：
• domain:The RING-type zinc finger domain is essential for ubiquitin ligase activity.,domain:The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family.,function:E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), cytoplasmic signal

• Subcellular Location：
• Cytoplasm. Nucleus. Predominantly cytoplasmic. Partially nuclear.

• Expression：
• Widely expressed at a low level. Down-regulated in advanced hepatocellular carcinomas.

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