• Target：
• Sam 68

• Gene Name：
• KHDRBS1

• Protein Name：
• KH domain-containing RNA-binding signal transduction-associated protein 1

• Human Gene Id：
• 10657

• Human Swiss Prot No：
• Q07666

• Mouse Gene Id：
• 20218

• Mouse Swiss Prot No：
• Q60749

• Rat Gene Id：
• 117268

• Rat Swiss Prot No：
• Q91V33

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human Sam 68. AA range:96-145

• Specificity：
• Sam 68 Polyclonal Antibody detects endogenous levels of Sam 68 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• KHDRBS1;SAM68;KH domain-containing; RNA-binding, signal transduction-associated protein 1;GAP-associated tyrosine phosphoprotein p62;Src-associated in mitosis 68 kDa protein;Sam68;p21 Ras GTPase-activating protein-associated p62;p68

• Observed Band(KD)：
• 68kD

• Background：
• This gene encodes a member of the K homology domain-containing, RNA-binding, signal transduction-associated protein family. The encoded protein appears to have many functions and may be involved in a variety of cellular processes, including alternative splicing, cell cycle regulation, RNA 3'-end formation, tumorigenesis, and regulation of human immunodeficiency virus gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012],

• Function：
• developmental stage:Isoform 3 is only expressed in growth-arrested cells.,domain:The KH domain is required for binding to RNA.,domain:The Pro-rich domains are flanked by Arg/Gly-rich motifs which can be asymmetric dimethylated on arginine residues to give the DMA/Gly-rich regions. Selective methlylation on these motifs can modulate protein-protein interactions.,function:Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase.,function:Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as

• Subcellular Location：
• Nucleus . Cytoplasm . Membrane . Predominantly located in the nucleus but also located partially in the cytoplasm. .

• Expression：
• Ubiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver.

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