• Target：
• Rit1

• Gene Name：
• RIT1

• Protein Name：
• GTP-binding protein Rit1

• Human Gene Id：
• 6016

• Human Swiss Prot No：
• Q92963

• Mouse Gene Id：
• 19769

• Mouse Swiss Prot No：
• P70426

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human RIT1. AA range:170-219

• Specificity：
• Rit1 Polyclonal Antibody detects endogenous levels of Rit1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• RIT1;RIBB;RIT;ROC1;GTP-binding protein Rit1;Ras-like protein expressed in many tissues;Ras-like without CAAX protein 1

• Observed Band(KD)：
• 25kD

• Background：
• This gene encodes a member of a subfamily of Ras-related GTPases. The encoded protein is involved in regulating p38 MAPK-dependent signaling cascades related to cellular stress. This protein also cooperates with nerve growth factor to promote neuronal development and regeneration. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012],

• Function：
• enzyme regulation:Alternate between an inactive form bound to GDP and an active form bound to GTP.,function:Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation.,miscellaneous:Shows rapid uncatalyzed guanine nucleotide dissociation rates, which are much faster than those of most Ras subfamily members.,miscellaneous:Stimulation of the NGF and EGF receptor signaling pathways results in rapid and prolonged activation.,similarity:Belongs to the small GTPase superfamily. Ras family.,subunit:Interacts with MLLT4, the C-terminal domain of RALGDS and RLF, but not with RIN1 and PIK3CA. RLF binds exclusively to the active GTP-bound form. Strongly interacts with BRAF, but only weakly with RAF1. BARF and RAF1 association is dependent upon the GTP-bound state. Interacts with RGL3.,tissue specificit

• Subcellular Location：
• Cell membrane.

• Expression：
• Expressed in many tissues.

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