Ribosomal Protein L12 Polyclonal Antibody
- Catalog No.:YT4095
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- Ribosomal Protein L12
- Fields:
- >>Ribosome;>>Coronavirus disease - COVID-19
- Gene Name:
- RPL12
- Protein Name:
- 60S ribosomal protein L12
- Human Gene Id:
- 6136
- Human Swiss Prot No:
- P30050
- Mouse Gene Id:
- 269261
- Mouse Swiss Prot No:
- P35979
- Rat Gene Id:
- 499782
- Rat Swiss Prot No:
- P23358
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human RPL12. AA range:61-110
- Specificity:
- Ribosomal Protein L12 Polyclonal Antibody detects endogenous levels of Ribosomal Protein L12 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- RPL12;60S ribosomal protein L12
- Observed Band(KD):
- 18kD
- Background:
- Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L11P family of ribosomal proteins. It is located in the cytoplasm. The protein binds directly to the 26S rRNA. This gene is co-transcribed with the U65 snoRNA, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008],
- Function:
- function:Binds directly to 26S ribosomal RNA.,similarity:Belongs to the ribosomal protein L11P family.,
- Subcellular Location:
- nucleolus,cytosol,focal adhesion,membrane,cytosolic large ribosomal subunit,extracellular matrix,extracellular exosome,
- Expression:
- Brain,Epithelium,Ovary,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using Ribosomal Protein L12 Polyclonal Antibody diluted at 1:2000
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from HepG2 and HeLa cells, using RPL12 Antibody. The lane on the right is blocked with the synthesized peptide.