Rabenosyn-5 Polyclonal Antibody

    • Catalog No.:YT3949
    • Applications:WB;ELISA;IHC
    • Reactivity:Human;Mouse
      • Target:
      • Rabenosyn-5
      • Fields:
      • >>Endocytosis
      • Gene Name:
      • ZFYVE20
      • Protein Name:
      • Rabenosyn-5
      • Human Swiss Prot No:
      • Q9H1K0
      • Mouse Swiss Prot No:
      • Q80Y56
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human ZFYVE20. AA range:145-194
      • Specificity:
      • Rabenosyn-5 Polyclonal Antibody detects endogenous levels of Rabenosyn-5 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • ZFYVE20;Rabenosyn-5;110 kDa protein;FYVE finger-containing Rab5 effector protein rabenosyn-5;Zinc finger FYVE domain-containing protein 20
      • Observed Band(KD):
      • 89kD
      • Background:
      • This gene encodes a protein that belongs to the FYVE zinc finger family of proteins. The encoded protein interacts with Ras-related proteins that regulate membrane trafficking. A missense mutation in this gene is associated with a defect in the early endocytic pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014],
      • Function:
      • function:Rab4/Rab5 effector protein acting in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Required for endosome fusion either homotypically or with clathrin coated vesicles. Plays a role in the lysosomal trafficking of cathepsin D from the Golgi to lysosomes. Also promotes the recycling of transferrin directly from early endosomes to the plasma membrane. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate (PtdInsP3).,similarity:Contains 1 C2H2-type zinc finger.,similarity:Contains 1 FYVE-type zinc finger.,similarity:Contains 1 UIM (ubiquitin-interacting motif) repeat.,subunit:Interacts with EHD1, RAB4A, RAB5A, RAB22A, RAB24 and VPS45. Binds simultaneously to RAB4A and RAB5A in vitro. Interacts with RAB4A and RAB5A that has been activated by GTP binding.,
      • Subcellular Location:
      • Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane ; Lipid-anchor. Enriched in endosomes that are in close proximity to clathrin-enriched regions at the cell surface. .
      • Expression:
      • Brain,Cervix carcinoma,Epithelium,Human salivary gland,
      • Products Images
      • Western Blot analysis of various cells using Rabenosyn-5 Polyclonal Antibody diluted at 1:1000
      • Western blot analysis of lysates from HUVEC and Jurkat cells, using ZFYVE20 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from COLO205 cells using ZFYVE20 antibody.
      • Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).