• Target：
• PXR

• Gene Name：
• NR1I2

• Protein Name：
• Nuclear receptor subfamily 1 group I member 2

• Human Gene Id：
• 8856

• Human Swiss Prot No：
• O75469

• Mouse Swiss Prot No：
• O54915

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human NR1I2. AA range:91-140

• Specificity：
• PXR Polyclonal Antibody detects endogenous levels of PXR protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NR1I2;PXR;Nuclear receptor subfamily 1 group I member 2;Orphan nuclear receptor PAR1;Orphan nuclear receptor PXR;Pregnane X receptor;Steroid and xenobiotic receptor;SXR

• Observed Band(KD)：
• 50kD

• Background：
• This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized. [provided by RefSeq, Jul 2008],

• Function：
• function:Orphan receptor; its natural ligand is probably pregnane. Binds to a response element in the CYP3A4 and ABCB1/MDR1 genes promoter. Activates its expression in response to a wide variety of endobiotics and xenobiotics.,induction:Activated by naturally occurring steroids such as pregnenolone and progesterone.,similarity:Belongs to the nuclear hormone receptor family. NR1 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,subunit:Forms a heterodimer with RXR.,tissue specificity:Expressed in liver, colon and small intestine.,

• Subcellular Location：
• Nucleus .

• Expression：
• Expressed in liver, colon and small intestine.

Imidacloprid increases intestinal permeability by disrupting tight junctions. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2021 Oct;222:112476 WB Rat 1:1000 Intestine

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