• Target：
• PUMA

• Fields：
• >>Platinum drug resistance;>>p53 signaling pathway;>>Apoptosis;>>Apoptosis - multiple species;>>Hippo signaling pathway;>>Huntington disease;>>Measles;>>Pathways in cancer;>>Colorectal cancer

• Gene Name：
• BBC3

• Protein Name：
• Bcl-2-binding component 3

• Human Gene Id：
• 27113

• Human Swiss Prot No：
• Q9BXH1

• Mouse Gene Id：
• 170770

• Mouse Swiss Prot No：
• Q99ML1

• Rat Gene Id：
• 317673

• Rat Swiss Prot No：
• Q80ZG6

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human PUMA. AA range:120-169

• Specificity：
• PUMA Polyclonal Antibody detects endogenous levels of PUMA protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• BBC3;PUMA;Bcl-2-binding component 3;JFY-1;p53 up-regulated modulator of apoptosis

• Observed Band(KD)：
• 23kD

• Background：
• This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011],

• Function：
• function:Essential mediator of p53-dependent and p53-independent apoptosis.,induction:By DNA damage, glucocorticoid treatment, growth factor deprivation and p53.,similarity:Belongs to the Bcl-2 family.,subcellular location:Localized to the mitochondria in order to induce cytochrome c release.,subunit:Interacts with MCL1 and BCL2A1 (By similarity). Interacts with BCL2 and BCL2L1/BCL-XL.,tissue specificity:Ubiquitously expressed.,

• Subcellular Location：
• Mitochondrion . Localized to the mitochondria in order to induce cytochrome c release.

• Expression：
• Ubiquitously expressed.

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