PKN Polyclonal Antibody

    • Catalog No.:YT3778
    • Applications:WB;IHC
    • Reactivity:Human;Mouse;Rat
      • Target:
      • PRK1
      • Fields:
      • >>PI3K-Akt signaling pathway;>>NOD-like receptor signaling pathway;>>Salmonella infection;>>Yersinia infection
      • Gene Name:
      • PKN1
      • Protein Name:
      • Serine/threonine-protein kinase N1
      • Human Gene Id:
      • 5585
      • Human Swiss Prot No:
      • Q16512
      • Mouse Swiss Prot No:
      • P70268
      • Immunogen:
      • Synthesized peptide derived from the Internal region of human PKN.
      • Specificity:
      • PKN Polyclonal Antibody detects endogenous levels of PKN protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500-2000;IHC-p 1:50-300
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • PKN1;PAK1;PKN;PRK1;PRKCL1;Serine/threonine-protein kinase N1;Protease-activated kinase 1;PAK-1;Protein kinase C-like 1;Protein kinase C-like PKN;Protein kinase PKN-alpha;Protein-kinase C-related kinase 1;Serine-threonine protein
      • Observed Band(KD):
      • 105kD
      • Background:
      • protein kinase N1(PKN1) Homo sapiens The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The C1 domain does not bind the diacylglycerol (DAG).,enzyme regulation:Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Two specific sites, Thr-774 (activation loop of the kinase domain) and Ser-916 (turn motif), need to be phosphorylated for its full activation.,function:Can phosphorylate ribosomal protein S6. Mediates GTPase Rho dependent intracellular signaling.,PTM:Activated by limited proteolysis with trypsin.,PTM:Autophosphorylated; preferably on serine.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 C2 domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 3 REM (
      • Subcellular Location:
      • Cytoplasm . Nucleus . Endosome . Cell membrane ; Peripheral membrane protein . Cleavage furrow . Midbody . Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-377 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. .
      • Expression:
      • Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells.
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).