• Target：
• PHLDA3

• Gene Name：
• PHLDA3

• Protein Name：
• Pleckstrin homology-like domain family A member 3

• Human Gene Id：
• 23612

• Human Swiss Prot No：
• Q9Y5J5

• Mouse Gene Id：
• 27280

• Mouse Swiss Prot No：
• Q9WV95

• Rat Gene Id：
• 363989

• Rat Swiss Prot No：
• Q5PQT7

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human PHLDA3. AA range:21-70

• Specificity：
• PHLDA3 Polyclonal Antibody detects endogenous levels of PHLDA3 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PHLDA3;TIH1;Pleckstrin homology-like domain family A member 3;TDAG51/Ipl homolog 1

• Observed Band(KD)：
• 15kD

• Background：
• domain:The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.,function:p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor.,induction:By p53/TP53; expression is directly activated by p53/TP53. p53/TP53 phosphorylation on 'Ser-15' is required to activate the PHLDA3 promoter.,miscellaneous:PHLDA3 genomic locus is frequently observed in primary lung cancers, suggesting a role in tumor suppression.,similarity:Belongs to the PHLDA3 family.,similarity:Contains 1 PH domain.,tissue specificity:Widely expressed with lowest expression in liver and spleen.,

• Function：
• domain:The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.,function:p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor.,induction:By p53/TP53; expression is directly activated by p53/TP53. p53/TP53 phosphorylation on 'Ser-15' is required to activate the PHLDA3 prom

• Subcellular Location：
• Cytoplasm . Membrane ; Peripheral membrane protein .

• Expression：
• Widely expressed with lowest expression in liver and spleen.

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