PHAPI2 Polyclonal Antibody

    • Catalog No.:YT3695
    • Applications:WB;ELISA;IHC
    • Reactivity:Human;Mouse;Rat
      • Target:
      • PHAPI2
      • Gene Name:
      • ANP32B
      • Protein Name:
      • Acidic leucine-rich nuclear phosphoprotein 32 family member B
      • Human Swiss Prot No:
      • Q92688
      • Mouse Swiss Prot No:
      • Q9EST5
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human ANP32B. AA range:1-50
      • Specificity:
      • PHAPI2 Polyclonal Antibody detects endogenous levels of PHAPI2 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • ANP32B;APRIL;PHAPI2;Acidic leucine-rich nuclear phosphoprotein 32 family member B;Acidic protein rich in leucines;Putative HLA-DR-associated protein I-2;PHAPI2;Silver-stainable protein SSP29
      • Observed Band(KD):
      • 29kD
      • Background:
      • function:Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity.,similarity:Belongs to the ANP32 family.,similarity:Contains 4 LRR (leucine-rich) repeats.,subcellular location:Accumulates in the nuclei at the S phase.,tissue specificity:Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta.,
      • Function:
      • function:Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity.,similarity:Belongs to the ANP32 family.,similarity:Contains 4 LRR (leucine-rich) repeats.,subcellular location:Accumulates in the nuclei at the S phase.,tissue specificity:Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta.,
      • Subcellular Location:
      • [Isoform 1]: Nucleus . Cytoplasm . Accumulates in the nuclei at the S phase. .; [Isoform 2]: Cytoplasm. Lacks a nuclear localization signal.
      • Expression:
      • Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta.
      • Products Images
      • Western Blot analysis of rat cells using PHAPI2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Western blot analysis of lysates from rat brain cells, using ANP32B Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from HeLa cells using ANP32B antibody.
      • Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).