• Target：
• PC-PLD3

• Fields：
• >>Glycerophospholipid metabolism;>>Ether lipid metabolism;>>Metabolic pathways

• Gene Name：
• PLD3

• Protein Name：
• Phospholipase D3

• Human Gene Id：
• 23646

• Human Swiss Prot No：
• Q8IV08

• Mouse Gene Id：
• 18807

• Mouse Swiss Prot No：
• O35405

• Rat Gene Id：
• 361527

• Rat Swiss Prot No：
• Q5FVH2

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human PLD3. AA range:326-375

• Specificity：
• PC-PLD3 Polyclonal Antibody detects endogenous levels of PC-PLD3 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PLD3;Phospholipase D3;PLD 3;Choline phosphatase 3;HindIII K4L homolog;Hu-K4;Phosphatidylcholine-hydrolyzing phospholipase D3

• Molecular Weight(Da)：
• 55kD

• Background：
• This gene encodes a member of the phospholipase D (PLD) family of enzymes that catalyze the hydrolysis of membrane phospholipids. The encoded protein is a single-pass type II membrane protein and contains two PLD phosphodiesterase domains. This protein influences processing of amyloid-beta precursor protein. Mutations in this gene are associated with Alzheimer disease risk. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Apr 2014],

• Function：
• catalytic activity:A phosphatidylcholine + H(2)O = choline + a phosphatidate.,PTM:Glycosylated.,similarity:Belongs to the phospholipase D family.,similarity:Contains 2 PLD phosphodiesterase domains.,tissue specificity:Widely expressed. Expressec at higher level in brain. Expresse at low level in corpus callosum, suggsting that it is highly expressed in neurons.,

• Subcellular Location：
• Endoplasmic reticulum membrane ; Single-pass type II membrane protein . Lysosome lumen . Early endosome membrane ; Single-pass type II membrane protein . Late endosome membrane ; Single-pass type II membrane protein . Golgi apparatus membrane ; Single-pass type II membrane protein . Endosome membrane ; Single-pass type II membrane protein . Localizes to ER-associated vesicles in differentiating myotubes (PubMed:22428023). The soluble form in lysosome arises by proteolytic processing of the membrane-bound form (PubMed:29386126). Colocalizes with APP in endosomes (PubMed:29368044). .

• Expression：
• Widely expressed. In the brain, high levels of expression are detected in the frontal, temporal and occipital cortices and hippocampus. Expressed at low level in corpus callosum.

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