PBFE Polyclonal Antibody
- Catalog No.:YT3610
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Rat
- Target:
- PBFE
- Fields:
- >>Fatty acid degradation;>>Valine, leucine and isoleucine degradation;>>Lysine degradation;>>Tryptophan metabolism;>>beta-Alanine metabolism;>>Propanoate metabolism;>>Butanoate metabolism;>>Metabolic pathways;>>Fatty acid metabolism;>>PPAR signaling pathway;>>Peroxisome
- Gene Name:
- EHHADH
- Protein Name:
- Peroxisomal bifunctional enzyme
- Human Gene Id:
- 1962
- Human Swiss Prot No:
- Q08426
- Mouse Swiss Prot No:
- Q9DBM2
- Rat Gene Id:
- 171142
- Rat Swiss Prot No:
- P07896
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human EHHADH. AA range:476-525
- Specificity:
- PBFE Polyclonal Antibody detects endogenous levels of PBFE protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- EHHADH;ECHD;Peroxisomal bifunctional enzyme;PBE;PBFE
- Observed Band(KD):
- 80kD
- Background:
- catalytic activity:(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O.,catalytic activity:(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.,catalytic activity:(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.,disease:Absent in patients suffering with peroxisomal disorders such as Zellweger syndrome, neonatal adrenoleukodystrophy and infantile Refsum disease.,pathway:Lipid metabolism; fatty acid beta-oxidation.,similarity:In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.,similarity:In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.,subunit:Monomer.,tissue specificity:Liver and kidney. Lower amounts seen in the brain.,
- Function:
- catalytic activity:(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O.,catalytic activity:(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.,catalytic activity:(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.,disease:Absent in patients suffering with peroxisomal disorders such as Zellweger syndrome, neonatal adrenoleukodystrophy and infantile Refsum disease.,pathway:Lipid metabolism; fatty acid beta-oxidation.,similarity:In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.,similarity:In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.,subunit:Monomer.,tissue specificity:Liver and kidney. Lower amounts seen in the brain.,
- Subcellular Location:
- Peroxisome .
- Expression:
- Liver and kidney. Strongly expressed in the terminal segments of the proximal tubule. Lower amounts seen in the brain.
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using PBFE Polyclonal Antibody
- Western Blot analysis of A549 cells using PBFE Polyclonal Antibody
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using EHHADH Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from A549 cells, using EHHADH Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HepG2 cells using EHHADH antibody.