• Target：
• p53RFP

• Gene Name：
• RNF144B

• Protein Name：
• E3 ubiquitin-protein ligase RNF144B

• Human Gene Id：
• 255488

• Human Swiss Prot No：
• Q7Z419

• Mouse Gene Id：
• 218215

• Mouse Swiss Prot No：
• Q8BKD6

• Immunogen：
• Synthesized peptide derived from the Internal region of human p53RFP.

• Specificity：
• p53RFP Polyclonal Antibody detects endogenous levels of p53RFP protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• RNF144B;IBRDC2;P53RFP;E3 ubiquitin-protein ligase RNF144B;IBR domain-containing protein 2;RING finger protein 144B;p53-inducible RING finger protein

• Observed Band(KD)：
• 35kD

• Background：
• caution:Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.,domain:The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.,function:E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism.,pathway:Protein modification; protein ubiquitination.,PTM:Auto-ubiquitinated.,similarity:Belongs to the RBR family. RNF144 subfamily.,similarity:Contains 1 IBR-type zinc finger.,similarity:Contains 2 RING-type zinc fingers.,subunit:Interacts with UBE2L3, UBE2L6 and LCMT2.,tissue specificity:Broadly expressed, with lowest levels in brain, spleen and thymus.,

• Function：
• caution:Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.,domain:The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.,function:E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism.,pathway:Protein modification; protein ubiquitination.,PTM:Auto-ubiquitinated.,similarity:Belongs to the RBR family. RNF144 subfamily.,similarity:Contains 1 IBR-type zinc finger.,similarity:Contains 2 RING-type zinc fingers.,subunit:Interacts with UBE2L3, UBE2L6 and LCMT2.,tissue specificity:Broadly expressed, with lowest levels in brain, spleen and thymu

• Subcellular Location：
• Mitochondrion membrane ; Single-pass membrane protein . Cytoplasm . Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation.

• Expression：
• Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.

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