• Target：
• Nrf2

• Fields：
• >>Protein processing in endoplasmic reticulum;>>Parkinson disease;>>Pathways in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Hepatocellular carcinoma;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis

• Gene Name：
• NFE2L2

• Protein Name：
• Nuclear factor erythroid 2-related factor 2

• Human Gene Id：
• 4780

• Human Swiss Prot No：
• Q16236

• Mouse Gene Id：
• 18024

• Mouse Swiss Prot No：
• Q60795

• Rat Gene Id：
• 83619

• Rat Swiss Prot No：
• O54968

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human Nrf2. AA range:556-605

• Specificity：
• Nrf2 Polyclonal Antibody detects endogenous levels of Nrf2 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NFE2L2;NRF2;Nuclear factor erythroid 2-related factor 2;NF-E2-related factor 2;NFE2-related factor 2;HEBP1;Nuclear factor; erythroid derived 2, like 2

• Observed Band(KD)：
• 75-100kD

• Background：
• This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015],

• Function：
• domain:Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.,function:Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.,PTM:Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. CNC subfamily.,similarity:Contains 1 bZIP domain.,subcellular location:Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electr

• Subcellular Location：
• Cytoplasm, cytosol . Nucleus . Cytosolic under unstressed conditions: ubiquitinated and degraded by the BCR(KEAP1) E3 ubiquitin ligase complex (PubMed:15601839, PubMed:21196497). Translocates into the nucleus upon induction by electrophilic agents that inactivate the BCR(KEAP1) E3 ubiquitin ligase complex (PubMed:21196497). .

• Expression：
• Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.

Therapeutic and Neuroprotective Effects of Bushen Jianpi Decoction on a Rotenone-Induced Rat Model of Parkinson’s Disease

Cadmium exposure promotes thyroid pyroptosis and endocrine dysfunction by inhibiting Nrf2/Keap1 signaling ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Yuying Chen, Chengcong Zhou, Yishan Bian, Fangda Fu, Bian'an Zhu, Xuyan Zhao, Muxin Zhang, Chunyuan Zhou, Sai Yao, Zhiguo Zhang, Huan Luo, Yuying Ge, Chengliang Wu, Hongfeng Ruan IF Mouse thyroid

An Integrated Approach Based on Network Analysis Combined With Experimental Verification Reveals PI3K/Akt/Nrf2 Signaling Is an Important Way for the Anti-Myocardial Ischemia Activity of Yi-Qi-Tong-Luo Capsule. Front Pharmacol. 2022 Feb;13:794528-794528. WB,IF Rat 1:1000

DJ-1 upregulates the Nrf2/GPX4 signal pathway to inhibit trophoblast ferroptosis in the pathogenesis of preeclampsia Sci Rep-Uk. 2022 Feb;12(1):1-16. WB Human 1:1000 Placenta

Genome-wide CRISPR/Cas9 library screening identified PHGDH as a critical driver for Sorafenib resistance in HCC. Nature Communications 2019 Oct 15 WB Human 1:500 MHCC97L cell

Photodriven nanoreactor with a hydrogen-insulin double act repairs diabetic wounds through Nrf2 pathway activation. CHEMICAL ENGINEERING JOURNAL Chem Eng J. 2021 Dec;425:131800 WB Mouse 1:1000 Skin RAW264.7 cells

The Keap1/Nrf2-ARE signaling pathway is involved in atrazine induced dopaminergic neurons degeneration via microglia activation. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2021 Dec;226:112862 WB Rat 1:1000 Neuron BV-2 cell

High Fat Diet-Induced Hepatic 18-Carbon Fatty Acids Accumulation Up-Regulates CYP2A5/CYP2A6 via NF-E2-Related Factor 2. Frontiers in Pharmacology Front Pharmacol. 2017 May;8:233 WB Human 1:200 HepG2 cell

Inhibition of ferroptosis by up-regulating Nrf2 delayed the progression of diabetic nephropathy. FREE RADICAL BIOLOGY AND MEDICINE Free Radical Bio Med. 2021 Jan;162:435 WB,IF Human,Mouse renal HK-2 cell

GSK-3β downregulates Nrf2 in cultured cortical neurons and in a rat model of cerebral ischemia-reperfusion. Scientific Reports Sci Rep-Uk. 2016 Feb;6(1):1-16 WB Rat 1:500 ischemic penumbra neurons

Active Peptide KF-8 from Rice Bran Attenuates Oxidative Stress in a Mouse Model of Aging Induced by d-Galactose. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY J Agr Food Chem. 2020;68(44):12271–12283 WB Mouse 1 : 1000 Liver NIH/3T3 cell

Angiotensin II-mediated improvement of renal mitochondrial function via the AMPK/PGC-1α/NRF-2 pathway is superior to norepinephrine in a rat model of septic shock associated with acute renal injury. Annals of Translational Medicine Ann Transl Med. 2021 Mar; 9(6): 481 WB Rat 1:1000 Renal cortical tissue

3,4-Dihydroxyphenylacetic acid, a microbiota-derived metabolite of quercetin, attenuates acetaminophen (APAP)-induced liver injury through activation of Nrf-2. XENOBIOTICA Xenobiotica. 2016;46(10):931-939 WB Mouse 1:1000 liver

Curcumin is a Potential Adjuvant to Alleviates Diabetic Retinal Injury via Reducing Oxidative Stress and Maintaining Nrf2 Pathway Homeostasis. Frontiers in Pharmacology Front Pharmacol. 2021 Dec;0:3696 WB Rat 1:1000 Retinal tissue

Modulations of Keap1-Nrf2 signaling axis by TIIA ameliorated the oxidative stress-induced myocardial apoptosis. FREE RADICAL BIOLOGY AND MEDICINE Free Radical Bio Med. 2018 Feb;115:191 WB,IF Mouse Myocardial Neonatal rat ventricular myocytes (NRVMs)

Protective effect of Potentilla anserina polysaccharide on cadmium-induced nephrotoxicity in vitro and in vivo. Food & Function Food Funct. 2017 Oct;8(10):3636-3646 WB Human,Mouse kidney tissues HEK293 cell

Artemisinin attenuates early renal damage on diabetic nephropathy rats through suppressing TGF-β1 regulator and activating the Nrf2 signaling pathway. LIFE SCIENCES Life Sci. 2020 Sep;256:117966 WB Rat Kidney

Glycogen Synthase Kinase 3β Influences Injury Following Cerebral Ischemia/Reperfusion in Rats. International Journal of Biological Sciences Int J Biol Sci. 2016; 12(5): 518–531 WB Rat 1:500 left cortical tissues neurons

Bu-Shen-Fang-Chuan formula attenuates cigarette smoke-induced inflammation by modulating the PI3K/Akt-Nrf2 and NF-κB signalling pathways. JOURNAL OF ETHNOPHARMACOLOGY J Ethnopharmacol. 2020 Oct;261:113095 WB，IHC Rat lung

Glutamine ameliorates intestinal ischemia-reperfusion Injury in rats by activating the Nrf2/Are signaling pathway. International Journal of Clinical and Experimental Pathology Int J Clin Exp Patho. 2015; 8(7): 7896–7904 IHC Rat 1:100 intestinal

MiR-125b attenuates retinal pigment epithelium oxidative damage via targeting Nrf2/HIF-1α signal pathway. EXPERIMENTAL CELL RESEARCH Exp Cell Res. 2022 Jan;410:112955 WB,IF Human 1:2000,1:200 ARPE-19 cell

JSH-23 prevents depressive-like behaviors in mice subjected to chronic mild stress: Effects on inflammation and antioxidant defense in the hippocampus. PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR Pharmacol Biochem Be. 2018 Jun;169:59 WB Mouse 1:500 Hippocampal

Dihydroartemisinin attenuates lipopolysaccharide‑induced acute lung injury in mice by suppressing NF‑κB signaling in an Nrf2‑dependent manner. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Int J Mol Med. 2019 Dec;44(6):2213-2222 WB Mouse 1:1000 lung tissues

The inflammation and reactive oxygen species regulated by Nrf2 and NF-κB signaling pathways in 630-nm light-emitting diode irradiation treated THP-1 monocytes/macrophages. LASERS IN MEDICAL SCIENCE 2020 Oct 31 WB Human 1:1000 THP-1 cell

Anti-Inflammatory and Antioxidant Effects of Kelong-Capsule on Testosterone-Induced Benign Prostatic Hyperplasia in Rats. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2018;2018:5290514 WB Rat 1 : 500 prostate tissue

Zhang, Hong, et al. "Therapeutic potential of bixin in PM2. 5 particles-induced lung injury in an Nrf2-dependent manner." Free Radical Biology and Medicine 126 (2018): 166-176.

Atef, Reham M., et al. "The Ying and Yang of Adenosine A 1 and A 2A Receptors on ERK1/2 Activation in a Rat Model of Global Cerebral Ischemia Reperfusion Injury." Molecular neurobiology 55.2 (2018): 1284-1298.

3, 4-Dihydroxyphenylacetic acid, a microbiota-derived metabolite of quercetin, attenuates acetaminophen (APAP)-induced liver injury through activation of Nrf-2." Xenobiotica 46.10 (2016): 931-939.

Correlation of In Vivo and In Vitro Assay Results for Assessment of Free Radical Scavenging Activity of Green Tea Nutraceuticals. JOURNAL OF FOOD SCIENCE J Food Sci. 2016 Jul;81(7):C1707-C1715 ELISA Rat hepatic

Role of Transcription Factor Nrf2 in Pyroptosis in Spinal Cord Injury by Regulating GSDMD NEUROCHEMICAL RESEARCH Yi Liao IF Rat

Paeonol protects against acute pancreatitis by Nrf2 and NF-κB pathways in mice JOURNAL OF PHARMACY AND PHARMACOLOGY Короткова Ирина Павловна WB Mouse

Berberine hydrochloride alleviates imatinib mesylate – induced cardiotoxicity through the inhibition of Nrf2-dependent ferroptosis Food & Function Jie Zhang IF,WB Rat H9c2 cells

SENP6-Mediated deSUMOylation of VEGFR2 Enhances Its Cell Membrane Transport in Angiogenesis INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Xiaohua Guo WB Human human umbilical vein endothelial cells (HUVECs)

New Insights into Baicalein’s Effect on Chlorpyrifos-Induced Liver Injury in Carp: Involving Macrophage Polarization and Pyropto sis JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY Shiwen Xu WB Fish liver tissue

GSK-3β-dependent Nrf2 antioxidant response modulates ferroptosis of lens epithelial cells in age-related cataract. Yu Qin WB Human 1:2000 HLE-B3 cell

To study the protective effect of Huangqi Baihe Granules on Radiation brain injury based on network pharmacology and experiment. Yongqi Liu WB Rat 1:1000 brain tissue

Itaconic acid induces angiogenesis and suppresses apoptosis via Nrf2/autophagy to prolong the survival of multi-territory perforator flaps. Liming Qing WB Rat 1:1000 flap tissue

AR/PCC herb pair inhibits osteoblast pyroptosis to alleviate diabetes-related osteoporosis by activating Nrf2/Keap1 pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Hongfeng Ruan IF Rat 1:500 vertebral body

Fullerol rescues the light-induced retinal damage by modulating Müller glia cell fate. Redox Biology Haiwei Xu IF，WB Human 1:200，1:1000 MIO-M1 cell

Protective effects of epigallocatechin-3-gallate counteracting the chronic hypobaric hypoxia-induced myocardial injury in plain-grown rats at high altitude. CELL STRESS & CHAPERONES Gao Fabao WB Rat 1:2000 left ventricle (LV) tissues

Melatonin protects photoreceptor cells against ferroptosis in dry AMD disorder by inhibiting GSK-3B/Fyn-dependent Nrf2 nuclear translocation BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE Xinyu Zhi WB,IF Mouse 1:2000 661W cell

Glycine recalibrates iron homeostasis of lens epithelial cells by blocking lysosome-dependent ferritin degradation FREE RADICAL BIOLOGY AND MEDICINE Ludi Wang IF,WB Mouse,Human 1:200,1:2000 eyeball HLE-B3 cell

Cepharanthine, a regulator of keap1-Nrf2, inhibits gastric cancer growth through oxidative stress and energy metabolism pathway Cell Death Discovery Lu Yang-yang IF Human,Mouse 1:200 AGS cell,HGC27 cell,MFC cell

Sunitinib induces cardiotoxicity through modulating oxidative stress and Nrf2-dependent ferroptosis in vitro and in vivo CHEMICO-BIOLOGICAL INTERACTIONS Dongjie Li IF,WB Mouse,Rat 1:200 heart tissue H9c2 cell

Gualou-Xiebai herb pair ameliorate atherosclerosis in HFD-induced ApoE−/− mice and inhibit the ox-LDL-induced injury of HUVECs by regulating the Nrf2-mediated ferroptosis JOURNAL OF ETHNOPHARMACOLOGY Li Zhu WB,IF Mouse,Human 1:5000 aortic tissue human vascular endothelial cells (HUVECs)

Nrf2-mediated redox balance alleviates LPS-induced vascular endothelial cell inflammation by inhibiting endothelial cell ferroptosis Scientific Reports Hou Huimin WB,IF Human 1:1000,1:200 Human umbilical vein endothelial cells (HUVEC)

Protective Effect of Mesenchymal Stem Cell Active Factor Combined with Alhagi maurorum Extract on Ulcerative Colitis and the Underlying Mechanism INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Xuanhong Cao WB Mouse 1:1200 colon tissue

Hypidone hydrochloride (YL-0919) ameliorates functional deficits after traumatic brain injury in mice by activating the sigma-1 receptor for antioxidation Neural Regeneration Research Bai Yafan WB,IF Mouse 1:1000 brain tissue

Anti-aging mechanism and effect of treatment with raw and winedwine-steamed Polygonatum sibiricumon about D-galactose-induced aging in mice by inhibiting oxidative stress and modulating gut microbiota Frontiers in Pharmacology Zhong Ruixue WB Mouse 1:2000 brain tissue

Epigallocatechin-3-gallate alleviates ethanol-induced endothelia cells injury partly through alteration of NF-κB translocation and activation of the Nrf2 signaling pathway BIOLOGICAL & PHARMACEUTICAL BULLETIN Jie Xu WB Human 1:5000 Human Umbilical Vein Endothelial Cell(HUVEC)

Combination therapy enhances efficacy and overcomes toxicity of metal-based anti-diabetic agent BRITISH JOURNAL OF PHARMACOLOGY Bing Shang WB Mouse pancreatic tissue

Puerarin ameliorates non-alcoholic fatty liver disease by inhibiting lipid metabolism through FMO5 Frontiers in Pharmacology Li Zhaoyi WB Mouse liver tissue

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