Nrf2 Polyclonal Antibody

    • Catalog No.:YT3189
    • Applications:WB,IHC-p,IF/ICC,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • NFE2L2
      • Protein Name:
      • Nuclear factor erythroid 2-related factor 2
      • Human Gene Id:
      • 4780
      • Human Swiss Prot No:
      • Q16236
      • Mouse Swiss Prot No:
      • Q60795
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human Nrf2. AA range:556-605
      • Specificity:
      • Nrf2 Polyclonal Antibody detects endogenous levels of Nrf2 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • NFE2L2; NRF2; Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor; erythroid derived 2, like 2
      • MolecularWeight(Da):
      • 75-100
      • Observed Band(KD):
      • 75-100
      • Background:
      • nuclear factor, erythroid 2 like 2(NFE2L2) Homo sapiens This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015],
      • Function:
      • domain:Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.,function:Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.,PTM:Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. CNC subfamily.,similarity:Contains 1 bZIP domain.,subcellular location:Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electr
      • Subcellular Location:
      • chromatin,nucleus,cytoplasm,centrosome,cytosol,plasma membrane,protein-DNA complex,
      • Expression:
      • Fetal liver,Lung,Melanoma,Myeloid leukemia cell,Uterus,

      Yan, Shi-Hai, et al. "Modulations of Keap1-Nrf2 signaling axis by TIIA ameliorated the oxidative stress-induced myocardial apoptosis." Free Radical Biology and Medicine 115 (2018): 191-201.

      Zhang, Hong, et al. "Therapeutic potential of bixin in PM2. 5 particles-induced lung injury in an Nrf2-dependent manner." Free Radical Biology and Medicine 126 (2018): 166-176.

      Atef, Reham M., et al. "The Ying and Yang of Adenosine A 1 and A 2A Receptors on ERK1/2 Activation in a Rat Model of Global Cerebral Ischemia Reperfusion Injury." Molecular neurobiology 55.2 (2018): 1284-1298.

      Li, Yixin, et al. "Glycogen synthase kinase 3β influences injury following cerebral ischemia/reperfusion in rats." International journal of biological sciences 12.5 (2016): 518.

      Abd‐ElSalam, Heba‐Alla H., et al. "Correlation of in vivo and in vitro assay results for assessment of free radical scavenging activity of green tea nutraceuticals." Journal of food science 81.7 (2016): C1707-C1715.

      Zhang, Ling, et al. "Anti-Inflammatory and Antioxidant Effects of Kelong-Capsule on Testosterone-Induced Benign Prostatic Hyperplasia in Rats." Evidence-Based Complementary and Alternative Medicine 2018 (2018).

      Xue, Huiting, et al. "3, 4-Dihydroxyphenylacetic acid, a microbiota-derived metabolite of quercetin, attenuates acetaminophen (APAP)-induced liver injury through activation of Nrf-2." Xenobiotica 46.10 (2016): 931-939.

      Wang, Ai-Li, et al. "Glutamine ameliorates intestinal ischemia-reperfusion Injury in rats by activating the Nrf2/Are signaling pathway." International journal of clinical and experimental pathology 8.7 (2015): 7896.

      • Products Images
      • Western Blot analysis of various cells using Nrf2 Polyclonal Antibody diluted at 1:1000
      • Western Blot analysis of HuvEc cells using Nrf2 Polyclonal Antibody diluted at 1:1000
      • Immunofluorescence analysis of HUVEC cells, using Nrf2 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Nrf2 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HUVEC cells, using Nrf2 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Li, Yixin, et al. "Glycogen synthase kinase 3β influences injury following cerebral ischemia/reperfusion in rats." International journal of biological sciences 12.5 (2016): 518.
      • Zhang, Ling, et al. "Anti-Inflammatory and Antioxidant Effects of Kelong-Capsule on Testosterone-Induced Benign Prostatic Hyperplasia in Rats." Evidence-Based Complementary and Alternative Medicine 2018 (2018).
      • Wang, Ai-Li, et al. "Glutamine ameliorates intestinal ischemia-reperfusion Injury in rats by activating the Nrf2/Are signaling pathway." International journal of clinical and experimental pathology 8.7 (2015): 7896.