• Target：
• NOS2/iNOS

• Fields：
• >>Arginine biosynthesis;>>Arginine and proline metabolism;>>Metabolic pathways;>>Calcium signaling pathway;>>HIF-1 signaling pathway;>>Peroxisome;>>Apelin signaling pathway;>>Relaxin signaling pathway;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>Pathways of neurodegeneration - multiple diseases;>>Pertussis;>>Leishmaniasis;>>Chagas disease;>>Toxoplasmosis;>>Amoebiasis;>>Tuberculosis;>>Pathways in cancer;>>Small cell lung cancer

• Gene Name：
• NOS2, INOS

• Protein Name：
• Nitric oxide synthase inducible

• Human Gene Id：
• 4843

• Human Swiss Prot No：
• P35228

• Mouse Gene Id：
• 18126

• Mouse Swiss Prot No：
• P29477

• Rat Gene Id：
• 24599

• Rat Swiss Prot No：
• Q06518

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human iNOS. AA range:117-166

• Specificity：
• NOS2 Polyclonal Antibody detects endogenous levels of NOS2 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NOS2;NOS2A;Nitric oxide synthase; inducible;Hepatocyte NOS;HEP-NOS;Inducible NO synthase;Inducible NOS;iNOS;NOS type II;Peptidyl-cysteine S-nitrosylase NOS2

• Observed Band(KD)：
• 131kD

• Background：
• Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity.,function:Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.,induction:By endotoxins and cytokines.,online information:Nitric oxide synthase entry,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like domain.,subunit:Homodimer. Bin

• Subcellular Location：
• Cytoplasm, cytosol . Localizes as discrete foci scattered throughout the cytosol and in the presence of SPSB1 and SPSB4, exhibits a more diffuse cytosolic localization. .

• Expression：
• Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Expressed in chondrocytes (PubMed:7504305).

Mechanical loading mitigates osteoarthritis symptoms by regulating the inflammatory microenvironment in a mouse model 2022 Feb 27. WB Mouse articular cartilage

MFG-E8 regulated by miR-99b-5p protects against osteoarthritis by targeting chondrocyte senescence and macrophage reprogramming via the NF-κB pathway. Cell Death & Disease Cell Death Dis. 2021 May;12(6):1-15 WB Mouse 1 : 1000 primary murine chondrocytes，RAW264.7 cell

PM2.5 induces autophagy-mediated cell death via NOS2 signaling in human bronchial epithelium cells. International Journal of Biological Sciences Int J Biol Sci. 2018; 14(5): 557–564 WB Human BEASE-2B cell

Regulation of ezrin tension by S-nitrosylation mediates non-small cell lung cancer invasion and metastasis. Theranostics Theranostics. 2019; 9(9): 2555–2571 WB Human NSCLC tissue

Baicalein inhibits non‐small‐cell lung cancer invasion and metastasis by reducing ezrin tension in inflammation microenvironment. CANCER SCIENCE Cancer Sci. 2020 Oct; 111(10): 3802–3812 WB Human A549 cell， H1299 cell

Ameliorating effects of 1,8-cineole on LPS-induced human umbilical vein endothelial cell injury by suppressing NF-κB signaling in vitro. EUROPEAN JOURNAL OF PHARMACOLOGY Eur J Pharmacol. 2016 Oct;789:195 WB Human 1:600 HUVECs

Zhu, Xiao-Ming, et al. "PM2. 5 induces autophagy-mediated cell death via NOS2 signaling in human bronchial epithelium cells." International journal of biological sciences 14.5 (2018): 557.

Baicalein inhibits non‐small‐cell lung cancer invasion and metastasis by reducing ezrin tension in inflammation microenvironment. CANCER SCIENCE Cancer Sci. 2020 Oct; 111(10): 3802–3812 WB Human A549 cell， H1299 cell

Chlamydia psittaci plasmid-encoded CPSIT_P7 induces macrophage polarization to enhance the antibacterial response through TLR4-mediated MAPK and NF-κB pathways BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH Chaoqun Chen WB Mouse

Euphohelides A–C, ent-Abietane-Type Norditerpene Lactones from Euphorbia helioscopia and Their Anti-Inflammatory Activities JOURNAL OF NATURAL PRODUCTS Kun Gao WB Mouse RAW264.7 cell

Mannosylated polydopamine nanoparticles alleviate radiation- induced pulmonary fibrosis by targeting M2 macrophages and inhibiting the TGF-β1/Smad3 signaling pathway. Yuhui Hao WB Mouse lung tissue

Activation of AHR by ITE improves cardiac remodelling and function in rats after myocardial infarction. ESC Heart Failure Dajun Chai IF Rat cardiac tissue

Uncovering the Multitarget Therapeutic Mechanism of Tong-Xie-Yao-Fang on Irritable Bowel Syndrome JOURNAL OF FOOD QUALITY Xiangxue Ma WB Rat 1:1000 colonic tissue

The macrophage polarization in allergic responses induced by tropomyosin of Macrobrachium nipponense in cell and murine models INTERNATIONAL IMMUNOPHARMACOLOGY Yanhai Xie WB Mouse 1:1000 jejunum tissue peritoneal cell

Gut microbiota promotes macrophage M1 polarization in hepatic sinusoidal obstruction syndrome via regulating intestinal barrier function mediated by butyrate Gut Microbes Si Zhao IF,WB Mouse,Human 1:100,1:1000 liver tissue

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs