Nicalin Polyclonal Antibody
- Catalog No.:YT3124
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- Nicalin
- Gene Name:
- NCLN
- Protein Name:
- Nicalin
- Human Gene Id:
- 56926
- Human Swiss Prot No:
- Q969V3
- Mouse Gene Id:
- 103425
- Mouse Swiss Prot No:
- Q8VCM8
- Rat Gene Id:
- 314648
- Rat Swiss Prot No:
- Q5XIA1
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human NCLN. AA range:474-523
- Specificity:
- Nicalin Polyclonal Antibody detects endogenous levels of Nicalin protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- NCLN;Nicalin;Nicastrin-like protein
- Observed Band(KD):
- 63kD
- Background:
- function:May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.,similarity:Belongs to the nicastrin family.,subunit:Interacts with NOMO2. Due to the strong similarity between NOMO1, NOMO2 and NOMO3, it probably also interacts with NOMO1 and NOMO3. Participates in a large protein complex, which is not related to the gamma-secretase complex.,tissue specificity:Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart.,
- Function:
- function:May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.,similarity:Belongs to the nicastrin family.,subunit:Interacts with NOMO2. Due to the strong similarity between NOMO1, NOMO2 and NOMO3, it probably also interacts with NOMO1 and NOMO3. Participates in a large protein complex, which is not related to the gamma-secretase complex.,tissue specificity:Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart.,
- Subcellular Location:
- Endoplasmic reticulum membrane ; Single-pass membrane protein .
- Expression:
- Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart.
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- Antibody-FAQs
- Products Images
- Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from HepG2 and HUVEC cells, using NCLN Antibody. The lane on the right is blocked with the synthesized peptide.