NF-YB Polyclonal Antibody

    • Catalog No.:YT3091
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • NF-YB
      • Fields:
      • >>Antigen processing and presentation;>>Tuberculosis;>>Human T-cell leukemia virus 1 infection
      • Gene Name:
      • NFYB
      • Protein Name:
      • Nuclear transcription factor Y subunit beta
      • Human Gene Id:
      • 4801
      • Human Swiss Prot No:
      • P25208
      • Mouse Swiss Prot No:
      • P63139
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human NFYB. AA range:1-50
      • Specificity:
      • NF-YB Polyclonal Antibody detects endogenous levels of NF-YB protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • NFYB;HAP3;Nuclear transcription factor Y subunit beta;CAAT box DNA-binding protein subunit B;Nuclear transcription factor Y subunit B;NF-YB
      • Observed Band(KD):
      • 29kD
      • Background:
      • The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008],
      • Function:
      • domain:Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.,function:Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.,similarity:Belongs to the NFYB/HAP3 subunit family.,subunit:Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding.,
      • Subcellular Location:
      • Nucleus.
      • Expression:
      • Urinary bladder,
      • Products Images
      • Western Blot analysis of various cells using NF-YB Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Western blot analysis of lysates from 293 cells, using NFYB Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).