NF-YB Polyclonal Antibody
- Catalog No.:YT3091
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- NF-YB
- Fields:
- >>Antigen processing and presentation;>>Tuberculosis;>>Human T-cell leukemia virus 1 infection
- Gene Name:
- NFYB
- Protein Name:
- Nuclear transcription factor Y subunit beta
- Human Gene Id:
- 4801
- Human Swiss Prot No:
- P25208
- Mouse Gene Id:
- 18045
- Mouse Swiss Prot No:
- P63139
- Rat Gene Id:
- 25336
- Rat Swiss Prot No:
- P63140
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human NFYB. AA range:1-50
- Specificity:
- NF-YB Polyclonal Antibody detects endogenous levels of NF-YB protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- NFYB;HAP3;Nuclear transcription factor Y subunit beta;CAAT box DNA-binding protein subunit B;Nuclear transcription factor Y subunit B;NF-YB
- Observed Band(KD):
- 29kD
- Background:
- The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008],
- Function:
- domain:Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.,function:Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.,similarity:Belongs to the NFYB/HAP3 subunit family.,subunit:Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding.,
- Subcellular Location:
- Nucleus.
- Expression:
- Urinary bladder,
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using NF-YB Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from 293 cells, using NFYB Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).