NF-1 Polyclonal Antibody
- Catalog No.:YT3078
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- NF-1
- Gene Name:
- NFIA
- Protein Name:
- Nuclear factor 1 A-type
- Human Gene Id:
- 4774/4781/4782/4784
- Human Swiss Prot No:
- Q12857/O00712/P08651/Q14938
- Mouse Gene Id:
- 18027/18028/18029/18032
- Rat Gene Id:
- 25492
- Rat Swiss Prot No:
- P09414
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human Nuclear Factor 1. AA range:11-60
- Specificity:
- NF-1 Polyclonal Antibody detects endogenous levels of NF-1 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- NFIA;KIAA1439;Nuclear factor 1 A-type;NF1-A;Nuclear factor 1/A;CCAAT-box-binding transcription factor;CTF;Nuclear factor I/A;NF-I/A;NFI-A;TGGCA-binding protein;NFIB;Nuclear factor 1 B-type;NF1-B;Nuclear factor 1/B;CCAAT-box-b
- Observed Band(KD):
- 55kD
- Background:
- This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
- Function:
- function:Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the CTF/NF-I family.,similarity:Contains 1 CTF/NF-I DNA-binding domain.,subunit:Binds DNA as a homodimer.,
- Subcellular Location:
- Nucleus.
- Expression:
- Brain,Epithelium,Skeletal muscle,Testis,
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from rat brain and HUVEC cells, using Nuclear Factor 1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).