NF-1 Polyclonal Antibody

    • Catalog No.:YT3078
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • NF-1
      • Gene Name:
      • NFIA
      • Protein Name:
      • Nuclear factor 1 A-type
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human Nuclear Factor 1. AA range:11-60
      • Specificity:
      • NF-1 Polyclonal Antibody detects endogenous levels of NF-1 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • NFIA;KIAA1439;Nuclear factor 1 A-type;NF1-A;Nuclear factor 1/A;CCAAT-box-binding transcription factor;CTF;Nuclear factor I/A;NF-I/A;NFI-A;TGGCA-binding protein;NFIB;Nuclear factor 1 B-type;NF1-B;Nuclear factor 1/B;CCAAT-box-b
      • Observed Band(KD):
      • 55kD
      • Background:
      • This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
      • Function:
      • function:Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the CTF/NF-I family.,similarity:Contains 1 CTF/NF-I DNA-binding domain.,subunit:Binds DNA as a homodimer.,
      • Subcellular Location:
      • Nucleus.
      • Expression:
      • Brain,Epithelium,Skeletal muscle,Testis,
      • Products Images
      • Western blot analysis of lysates from rat brain and HUVEC cells, using Nuclear Factor 1 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).