• Target：
• N-cadherin

• Fields：
• >>Cell adhesion molecules;>>Arrhythmogenic right ventricular cardiomyopathy

• Gene Name：
• CDH2

• Protein Name：
• Cadherin-2

• Human Gene Id：
• 1000

• Human Swiss Prot No：
• P19022

• Mouse Gene Id：
• 12558

• Mouse Swiss Prot No：
• P15116

• Rat Gene Id：
• 83501

• Rat Swiss Prot No：
• Q9Z1Y3

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human CDH2. AA range:721-770

• Specificity：
• N-cadherin Polyclonal Antibody detects endogenous levels of N-cadherin protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• CDH2;CDHN;NCAD;Cadherin-2;CDw325;Neural cadherin;N-cadherin;CD antigen CD325

• Observed Band(KD)：
• 100-140kD

• Background：
• This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015],

• Function：
• function:Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism.,similarity:Contains 5 cadherin domains.,subunit:Interacts with CDCP1.,

• Subcellular Location：
• Cell membrane ; Single-pass type I membrane protein . Cell membrane, sarcolemma . Cell junction . Cell surface . Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes. .

• Expression：
• Brain,Epithelium,Liver,

Afatinib Reverses EMT via Inhibiting CD44-Stat3 Axis to Promote Radiosensitivity in Nasopharyngeal Carcinoma Pharmaceuticals Huichao Huang, Fangling Huang, Xujun Liang, Ying Fu, Zhe Cheng, Yan Huang, Zhuchu Chen, Yankun Duan, Yongheng Chen WB Human 5-8F cell, HNE2 cell

hsa_circ_0001741 promotes esophageal squamous cell carcinoma stemness, invasion and migration by sponging miR-491-5p to upregulate NOTCH3 expression WB Human,Mouse xenograft/ECA109 cell，TE1 cell

Promoter methylation-regulated miR-148a-3p inhibits lung adenocarcinoma (LUAD) progression by targeting MAP3K9. ACTA PHARMACOLOGICA SINICA2022 Apr;:1-10. Human 1:1000 A549 cell, NCI-H1299 cell

Licoflavone A Suppresses Gastric Cancer Growth and Metastasis by Blocking the VEGFR-2 Signaling Pathway. Journal of Oncology2022;2022:5497991. Human 1 : 1000 MKN-45 cell

A connexin43/YAP axis regulates astroglial-mesenchymal transition in hemoglobin induced astrocyte activation. CELL DEATH AND DIFFERENTIATION 2018 Jun 07 WB Rat 1:1000 Astrocytes

SREBP1, targeted by miR-18a-5p, modulates epithelial-mesenchymal transition in breast cancer via forming a co-repressor complex with Snail and HDAC1/2. CELL DEATH AND DIFFERENTIATION 2018 Jul 09 IHC Human breast cancer tissue

Tanshinone IIA mediates SMAD7-YAP interaction to inhibit liver cancer growth by inactivating the transforming growth factor beta signaling pathway. Aging-US Aging-Us. 2019 Nov 15; 11(21): 9719–9737 WB Human 1:2000 Bel-7404 cell, SMMC-7721 cell

N6-Methyladenosine Modification of CDH1 mRNA Promotes PM2.5-Induced Pulmonary Fibrosis via Mediating Epithelial Mesenchymal Transition. TOXICOLOGICAL SCIENCES Toxicol Sci. 2021 Nov;: WB Mouse,Human 1:1000 Lung BEAS-2B cell

Downregulation of RIPK4 Expression Inhibits Epithelial-Mesenchymal Transition in Ovarian Cancer through IL-6. Journal of Immunology Research J Immunol Res. 2021;2021:8875450 WB Human 1 : 1000 SKOV3 cell

Integration of exosomal miR-106a and mesothelial cells facilitates gastric cancer peritoneal dissemination. CELLULAR SIGNALLING Cell Signal. 2022 Mar;91:110230 WB Human 1:1000 HMrSV5 cell

Mesenchymal stem cell‑derived extracellular vesicles promote the in vitro proliferation and migration of breast cancer cells through the activation of the ERK pathway. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2019 May;54(5):1843-1852 WB Human 1:1000 hUC-MSC-EVs

Huaier Restrains Proliferative and Migratory Potential of Hepatocellular Carcinoma Cells Partially Through Decreased Yes-Associated Protein 1. Journal of Cancer J Cancer. 2017; 8(19): 4087–4097 WB Human Bel-7404 cell,SMMC-7721 cell

LncRNA GAS6-AS1 facilitates tumorigenesis and metastasis of colorectal cancer by regulating TRIM14 through miR-370-3p/miR-1296-5p and FUS Journal of Translational Medicine Jie Wu WB Human

The reversion of DNA methylation-induced miRNA silence via biomimetic nanoparticles-mediated gene delivery for efficient lung adenocarcinoma therapy Molecular Cancer Xiyong Yu WB Human

circRNAs deregulation in exosomes derived from BEAS-2B cells is associated with vascular stiffness induced by PM2.5 Journal of Environmental Sciences Rong Zhang WB Human human bronchial epithelial cell line (BEAS-2B)

Nanocarrier of Pin1 inhibitor based on supercritical fluid technology inhibits cancer metastasis by blocking multiple signaling pathways Regenerative Biomaterials Dayun Yang WB,IHC Human,Mouse lung tissues HuH7 cell,HepG2 cell

NMT1 sustains ICAM-1 to modulate adhesion and migration of tumor cells. Yongxia Qiao WB Human 1:500 Bel-7402 cell,Bel-7404 cell

Bufalin inhibits the proliferation of lung cancer cells by suppressing Hippo-YAP pathway. Yongchun Yu WB Human A549 cell

The combination of inositol hexaphosphate and inositol inhibits metastasis of colorectal cancer cells by upregulating claudin 7. Yang Song WB Human SW480 cell,SW620 cell

Tangeretin attenuates bleomycin-induced pulmonary fibrosis by inhibiting epithelial-mesenchymal transition via the PI3K/Akt pathway. Frontiers in Pharmacology Pengfei Yi WB Human，Mouse lung tissue A549 cell

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