N4BP1 Polyclonal Antibody
- Catalog No.：YT2962
- Applications：WB;IHC;IF;ELISA
- Reactivity：Human;Rat;Mouse;
- Target：
- N4BP1
- Gene Name：
- N4BP1
- Protein Name：
- NEDD4-binding protein 1
- Human Gene Id：
- 9683
- Human Swiss Prot No：
- O75113
- Mouse Swiss Prot No：
- Q6A037
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human N4BP1. AA range:383-432
- Specificity：
- N4BP1 Polyclonal Antibody detects endogenous levels of N4BP1 protein.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Polyclonal, Rabbit,IgG
- Dilution：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- N4BP1;KIAA0615;NEDD4-binding protein 1;N4BP1
- Observed Band(KD)：
- 100kD
- Background：
- PTM:Monoubiquitinated.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the N4BP1 family.,subcellular location:In discrete circular structures.,subunit:Binds NEDD4.,tissue specificity:Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.,
- Function：
- PTM:Monoubiquitinated.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the N4BP1 family.,subcellular location:In discrete circular structures.,subunit:Binds NEDD4.,tissue specificity:Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.,
- Subcellular Location：
- Cytoplasm, cytosol . Nucleus . Nucleus, nucleolus . Nucleus, PML body . Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated. .
- Expression：
- Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.
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- WESTERN IMMUNOBLOTTING PROTOCOL
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- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
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- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using N4BP1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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- Western Blot analysis of K562 cells using N4BP1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from MCF-7 and Jurkat/293 cells, using N4BP1 Antibody. The lane on the right is blocked with the synthesized peptide.
