• Target：
• N/H/K-Ras

• Fields：
• >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>Chemokine signaling pathway;>>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Mitophagy - animal;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Axon guidance;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Gap junction;>>Signaling pathways regulating pluripotency of stem cells;>>C-type lectin receptor signaling pathway;>>Natural killer cell mediated cytotoxicity;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>Thermogenesis;>>Long-term potentiation;>>Neurotrophin signaling pathway;>>Cholinergic synapse;>>Serotonergic synapse;>>Long-term depression;>>Regulation of actin

• Gene Name：
• NRAS/HRAS/KRAS

• Protein Name：
• GTPase Nras/GTPase Hras/GTPase Kras

• Human Gene Id：
• 3265/3845/4893

• Human Swiss Prot No：
• P01111/P01112/P01116

• Mouse Gene Id：
• 15461/16653

• Rat Gene Id：
• 24605/293621/24525

• Rat Swiss Prot No：
• Q04970/P20171/P08644

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human RASH/RASK. AA range:1-50

• Specificity：
• N/H/K-Ras Polyclonal Antibody detects endogenous levels of N/H/K-Ras protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NRAS;HRAS1;GTPase NRas;Transforming protein N-Ras;HRAS;HRAS1;GTPase HRas;H-Ras-1;Ha-Ras;Transforming protein p21;c-H-ras;p21ras;KRAS;KRAS2;RASK2;GTPase KRas;K-Ras 2;Ki-Ras;c-K-ras;c-Ki-ras

• Observed Band(KD)：
• 21kD

• Background：
• This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011],

• Function：
• disease:Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.,disease:Mutations which change AA 12, 13 or 61 activate the potential of Ras to transform cultured cells and are implicated in a variety of human tumors.,enzyme regulation:Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).,function:Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.,online information:NRAS mutation db,online information:RAS proteins entry,PTM:Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membran

• Subcellular Location：
• Cell membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane ; Lipid-anchor . Shuttles between the plasma membrane and the Golgi apparatus. .

• Expression：
• Bone marrow,Bone-marrow,Brain,Fibrosarcoma,Kidney,Leukemia,Lung car

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