Myp Polyclonal Antibody

    • Catalog No.:YT2953
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Rat;Mouse;
      • Target:
      • Myp
      • Gene Name:
      • NOL3
      • Protein Name:
      • Nucleolar protein 3
      • Human Gene Id:
      • 8996
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human ARC. AA range:159-208
      • Specificity:
      • Myp Polyclonal Antibody detects endogenous levels of Myp protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • Myp;NOL3;Nop30;Nucleolar protein 3;apoptosis repressor ARC;apoptosis repressor with CARD;apoptosis repressor with caspase recruitment domain (CARD);muscle-enriched cytoplasmic protein;nucleolar protein of 30 kDa
      • Observed Band(KD):
      • 28kD
      • Background:
      • NOL3 encodes an anti-apoptotic protein nucleolar protein 3 that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for NOL3.
      • Subcellular Location:
      • [Isoform 1]: Nucleus, nucleolus . The SR-rich C-terminus mediates nuclear localization. .; [Isoform 3]: Cytoplasm .; [Isoform 2]: Cytoplasm . Mitochondrion . Sarcoplasmic reticulum . Membrane ; Lipid-anchor . Phosphorylation at Thr-149 results in translocation to mitochondria. Colocalized with mitochondria in response to oxidative stress. .
      • Expression:
      • Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.
      • Products Images
      • Western Blot analysis of various cells using Myp Polyclonal Antibody
      • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ARC Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HeLa cells, using ARC Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western Blot analysis of lane1 mouse-brain, lane2 mouse-kidney, lane3 Hela. land4 MCF7, lane5 293T, lane6 mouse-muscle using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 25°C,1.5hours