• Target：
• MyD88

• Fields：
• >>MAPK signaling pathway;>>NF-kappa B signaling pathway;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>Alcoholic liver disease;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Pertussis;>>Legionellosis;>>Yersinia infection;>>Leishmaniasis;>>Chagas disease;>>African trypanosomiasis;>>Malaria;>>Toxoplasmosis;>>Tuberculosis;>>Hepatitis B;>>Measles;>>Influenza A;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Coronavirus disease - COVID-19;>>PD-L1 expression and PD-1 checkpoint pathway in cancer;>>Lipid and atherosclerosis

• Gene Name：
• MyD88

• Protein Name：
• Myeloid differentiation primary response protein MyD88

• Human Gene Id：
• 4615

• Human Swiss Prot No：
• Q99836

• Mouse Gene Id：
• 17874

• Mouse Swiss Prot No：
• P22366

• Rat Gene Id：
• 301059

• Rat Swiss Prot No：
• Q6Y1S1

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human MyD88. AA range:171-220

• Specificity：
• MyD88 Polyclonal Antibody detects endogenous levels of MyD88 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunocytochemistry: 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MYD88;Myeloid differentiation primary response protein MyD88

• Observed Band(KD)：
• 33kD

• Background：
• This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010],

• Function：
• disease:Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also called recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.,function:Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. May be involved in myeloid differentiation.,similarity:Contains 1 death domain.,similarity:Contains 1 TIR domain.,subunit:Homodimer. Also forms hetero

• Subcellular Location：
• Cytoplasm . Nucleus .

• Expression：
• Ubiquitous.

Crocin alleviates cognitive impairment associated with atherosclerosis via improving neuroinflammation in LDLR−/− mice fed a high-fat/cholesterol diet 2022 Jan 27. WB Mouse 1:1000 hippocampus,parietal cortex

Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 Pathway. Frontiers in Pharmacology Front Pharmacol. 2021; 12: 755140 WB Rat Vessels tissue

Sucralose enhances the susceptibility to dextran sulfate sodium (DSS) induced colitis in mice with changes in gut microbiota. Food & Function Food Funct. 2021 Oct;12(19):9380-9390 WB Mouse Colonic tissue

7-Difluoromethoxy-5,4′-dimethoxy-genistein attenuates macrophages apoptosis to promote plaque stability via TIPE2/TLR4 axis in high fat diet-fed ApoE−/− mice. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2021 Jul;96:107477 WB Mouse 1 : 2000 RAW264.7 cell

Dibromoacetic Acid Induced Hepatotoxicity in Mice through Oxidative Stress and Toll-Like Receptor 4 Signaling Pathway Activation. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2019;2019:5637235 WB Mouse Liver

Oat fiber supplementation alleviates intestinal inflammation and ameliorates intestinal mucosal barrier via acting on gut microbiota-derived metabolites in LDLR–/– mice. NUTRITION Nutrition. 2022 Mar;95:111558 WB Mouse Aortic tissue，intestinal tissue

Cereal Fiber Ameliorates High-Fat/Cholesterol-Diet-Induced Atherosclerosis by Modulating the NLRP3 Inflammasome Pathway in ApoE–/– Mice. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY J Agr Food Chem. 2018;66(19):4827–4834 WB Mouse 1:1000 aortas

Immunological effects of different types of synthetic CpG oligodeoxynucleotides on porcine cells. RSC Advances Rsc Adv. 2017 Sep;7(68):43289-43299 WB Pig 1:2000 IPEC-J2 cell

Establishment and Mechanism Study of a Primary Ovarian Insufficiency Mouse Model Using Lipopolysaccharide. Analytical Cellular Pathology Anal Cell Pathol. 2021;2021:1781532 WB Mouse 1:1000 Ovarian tissue

Anti-Oxidative and Immuno-Protective Effect of Camel Milk on Radiation-Induced Intestinal Injury in C57BL/6 J Mice:. Dose-Response Dose-Response. 2021;19(1): WB Mouse 1 : 1000 Jejunum

7-Difluoromethoxy-5, 4′-dimethoxy-genistein attenuates macrophages apoptosis to promote plaque stability via TIPE2/TLR4 axis in high fat diet-fed ApoE−/− mice." International Immunopharmacology 96 (2021): 107477.

Establishment and Mechanism Study of a Primary Ovarian Insufficiency Mouse Model Using Lipopolysaccharide. Analytical Cellular Pathology Anal Cell Pathol. 2021;2021:1781532 WB Mouse 1:1000 Ovarian tissue

The beneficial effects of Polygonatum sibiricum Red. superfine powder on metabolic hypertensive rats via gut-derived LPS/TLR4 pathway inhibition PHYTOMEDICINE Jingjing Yu WB,IHC Rat

Hydroxychloroquine protects against autoimmune premature ovarian insufficiency by modulating the Treg/Th17 cell ratio in BALB/c mice AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY Liping Wang WB Mouse splenocytes Treg/Th17 cell

Eif2s3y alleviated LPS-induced damage to mouse testis and maintained spermatogenesis by negatively regulating Adamts5. THERIOGENOLOGY Jinlian Hua WB Mouse 1:1000 testes

Salvianolic acid A alleviates heart failure with preserved ejection fraction via regulating TLR/Myd88/TRAF/NF-κB and p38MAPK/CREB signaling pathways BIOMEDICINE & PHARMACOTHERAPY Awaguli Dawuti WB Mouse ventricle tissue

Galectin from Trichinella spiralis alleviates DSS-induced colitis in mice by regulating the intestinal microbiota VETERINARY RESEARCH Li Jianqing WB Mouse 1:1000 colon tissue

Puerarin suppresses macrophage M1 polarization to alleviate renal inflammatory injury through antagonizing TLR4/MyD88-mediated NF-κB p65 and JNK/FoxO1 activation PHYTOMEDICINE Zujian Hu Co-IP Mouse Raw264.7 cell

Integrating network pharmacology and experimental models to investigate the mechanisms of XCHD and YCSLS in preventing HUA progression via TLR4/MYD88/NF-κB signaling Heliyon Yining Luo WB Rat serum

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