MKP-7 Polyclonal Antibody
- Catalog No.:YT2777
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse
- Target:
- MKP-7
- Fields:
- >>MAPK signaling pathway
- Gene Name:
- DUSP16
- Protein Name:
- Dual specificity protein phosphatase 16
- Human Gene Id:
- 80824
- Human Swiss Prot No:
- Q9BY84
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human DUSP16. AA range:571-620
- Specificity:
- MKP-7 Polyclonal Antibody detects endogenous levels of MKP-7 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- DUSP16;KIAA1700;MKP7;Dual specificity protein phosphatase 16;Mitogen-activated protein kinase phosphatase 7;MAP kinase phosphatase 7;MKP-7
- Observed Band(KD):
- 73kD
- Background:
- dual specificity phosphatase 16(DUSP16) Homo sapiens This gene encodes a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. The encoded protein specifically regulates the c-Jun amino-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) pathways.[provided by RefSeq, May 2010],
- Function:
- catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,function:Involved in the inactivation of MAP kinases.,similarity:Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.,similarity:Contains 1 rhodanese domain.,similarity:Contains 1 tyrosine-protein phosphatase domain.,
- Subcellular Location:
- Cytoplasm. Nucleus. Cytoplasmic vesicle. After dissociation upon AGTR stimulation, re-associates with ARRB2 on endocytic vesicles.
- Expression:
- Bone marrow,Brain,Duodenum,PCR rescued clones,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Immunofluorescence analysis of HepG2 cells, using DUSP16 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from Jurkat and COLO205 cells, using DUSP16 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).