• Target：
• MEK Kinase-3

• Fields：
• >>MAPK signaling pathway;>>Neurotrophin signaling pathway;>>GnRH signaling pathway;>>Human T-cell leukemia virus 1 infection;>>PD-L1 expression and PD-1 checkpoint pathway in cancer

• Gene Name：
• MAP3K3

• Protein Name：
• Mitogen-activated protein kinase kinase kinase 3

• Human Gene Id：
• 4215

• Human Swiss Prot No：
• Q99759

• Mouse Gene Id：
• 26406

• Mouse Swiss Prot No：
• Q61084

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human MAP3K3. AA range:101-150

• Specificity：
• MEK Kinase-3 Polyclonal Antibody detects endogenous levels of MEK Kinase-3 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MAP3K3;MAPKKK3;MEKK3;Mitogen-activated protein kinase kinase kinase 3;MAPK/ERK kinase kinase 3;MEK kinase 3;MEKK 3

• Observed Band(KD)：
• 71kD

• Background：
• This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation on Thr-530.,function:Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 OPR domain.,similarity:Contains 1 protein kinase domain.,subunit:Binds both upstream activators and downstream substrates in multimolecular complexes. Part of a complex with MAP2K3, RAC1 and CCM2. Interacts with MAP2K5 and SPAG9.,

• Subcellular Location：
• cytoplasm,cytosol,

• Expression：
• Aorta,Brain,Epithelium,Kidney,Melanoma,Placenta,PNS

Endothelial hyperactivation of mutant MAP3K3 induces cerebral cavernous malformation enhanced by PIK3CA GOF mutation ANGIOGENESIS Yong Cao IF Mouse brain

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