• Target：
• ME3

• Fields：
• >>Pyruvate metabolism;>>Metabolic pathways;>>Carbon metabolism;>>PPAR signaling pathway

• Gene Name：
• ME3

• Protein Name：
• NADP-dependent malic enzyme mitochondrial

• Human Gene Id：
• 10873

• Human Swiss Prot No：
• Q16798

• Mouse Gene Id：
• 109264

• Mouse Swiss Prot No：
• Q8BMF3

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human ME3. AA range:545-594

• Specificity：
• ME3 Polyclonal Antibody detects endogenous levels of ME3 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ME3;NADP-dependent malic enzyme; mitochondrial;NADP-ME;Malic enzyme 3

• Observed Band(KD)：
• 67kD

• Background：
• Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH.,cofactor:Divalent metal cations. Prefers magnesium or manganese.,similarity:Belongs to the malic enzymes family.,tissue specificity:Expressed predominantly in organs with a low-division rate.,

• Subcellular Location：
• Mitochondrion matrix.

• Expression：
• Expressed predominantly in organs with a low-division rate.

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