Mcl-1 Polyclonal Antibody

    • Catalog No.:YT2679
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • Mcl-1
      • Fields:
      • >>PI3K-Akt signaling pathway;>>Apoptosis;>>JAK-STAT signaling pathway;>>MicroRNAs in cancer
      • Gene Name:
      • MCL1
      • Protein Name:
      • Induced myeloid leukemia cell differentiation protein Mcl-1
      • Human Gene Id:
      • 4170
      • Human Swiss Prot No:
      • Q07820
      • Mouse Swiss Prot No:
      • P97287
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human MCL1. AA range:91-140
      • Specificity:
      • Mcl-1 Polyclonal Antibody detects endogenous levels of Mcl-1 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • MCL1;BCL2L3;Induced myeloid leukemia cell differentiation protein Mcl-1;Bcl-2-like protein 3;Bcl2-L-3;Bcl-2-related protein EAT/mcl1;mcl1/EAT
      • Observed Band(KD):
      • About 40kd in human,39kd in mouse and rat
      • Background:
      • This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010],
      • Function:
      • function:Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis while isoform 2 promotes it.,induction:Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML-1. Rapidly up-regulated by CSF2 in ML-1 cells. Up-regulated by heat-shock induced differentiation. Expression increases early during retinoic acid-induced differentiation.,PTM:Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.,PTM:Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.,PTM:Rapidly degraded in the abs
      • Subcellular Location:
      • Membrane ; Single-pass membrane protein . Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Cytoplasmic, associated with mitochondria.
      • Expression:
      • Ewing sarcoma,Mammary gland,Myeloid leukemia cell,Neuroblastoma,Placenta,Th
      • Products Images
      • Chen, Puxiang, et al. "Long noncoding RNA LINC00152 promotes cell proliferation through competitively binding endogenous miR‐125b with MCL‐1 by regulating mitochondrial apoptosis pathways in ovarian cancer." Cancer medicine 7.9 (2018): 4530-4541.
      • Western Blot analysis of various cells using Mcl-1 Polyclonal Antibody diluted at 1:1000
      • Western Blot analysis of Jurkat cells using Mcl-1 Polyclonal Antibody diluted at 1:1000
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MCL1 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HUVEC cells, using MCL1 Antibody. The lane on the right is blocked with the synthesized peptide.