• Target：
• MARCH5

• Gene Name：
• MARCH5

• Protein Name：
• E3 ubiquitin-protein ligase MARCH5

• Human Gene Id：
• 54708

• Human Swiss Prot No：
• Q9NX47

• Mouse Gene Id：
• 69104

• Mouse Swiss Prot No：
• Q3KNM2

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human MARCH5. AA range:21-70

• Specificity：
• MARCH5 Polyclonal Antibody detects endogenous levels of 40607 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000 IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MARCH5;RNF153;E3 ubiquitin-protein ligase MARCH5;Membrane-associated RING finger protein 5;Membrane-associated RING-CH protein V;MARCH-V;Mitochondrial ubiquitin ligase;MITOL;RING finger protein 153

• Observed Band(KD)：
• 37kD

• Background：
• MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM, Mar 2008],

• Function：
• domain:The RING-CH-type zinc finger domain is required for E3 ligase activity.,function:Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology. Promotes ubiquitination of DRP1. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 RING-CH-type zinc finger.,subunit:Interacts with MTFN2 and ubiquitinated forms of DRP1.,

• Subcellular Location：
• Mitochondrion outer membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria (PubMed:16874301). .

• Expression：
• Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle.

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