• Target：
• MAN1B1

• Fields：
• >>N-Glycan biosynthesis;>>Various types of N-glycan biosynthesis;>>Metabolic pathways;>>Protein processing in endoplasmic reticulum

• Gene Name：
• MAN1B1

• Protein Name：
• Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase

• Human Gene Id：
• 11253

• Human Swiss Prot No：
• Q9UKM7

• Mouse Swiss Prot No：
• A2AJ15

• Immunogen：
• Synthesized peptide derived from MAN1B1 . at AA range: 100-180

• Specificity：
• MAN1B1 Polyclonal Antibody detects endogenous levels of MAN1B1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MAN1B1;Endoplasmic reticulum mannosyl-oligosaccharide 1;2-alpha-mannosidase;ER alpha-1,2-mannosidase;ER mannosidase 1;ERMan1;Man9GlcNAc2-specific-processing alpha-mannosidase;Mannosidase alpha class 1B member 1

• Observed Band(KD)：
• 80kD

• Background：
• This gene encodes an enzyme belonging to the glycosyl hydrolase 47 family. This enzyme functions in N-glycan biosynthesis, and is a class I alpha-1,2-mannosidase that specifically converts Man9GlcNAc to Man8GlcNAc isomer B. It is required for N-glycan trimming to Man5-6GlcNAc2 in the endoplasmic-reticulum-associated degradation pathway. Mutations in this gene cause autosomal-recessive intellectual disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. [provided by RefSeq, Dec 2011],

• Function：
• catalytic activity:Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man(9)(GlcNAc)(2).,cofactor:Calcium.,enzyme regulation:Inhibited by both 1-deoxymannojirimycin and kifunensine.,function:Involved in the maturation of Asn-linked oligosaccharides. Trim a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2). The only product is the Man(8)GlcNAc(2) isomer B, the form lacking the middle-arm terminal alpha 1,2-mannose. It may be involved in glycoprotein quality control since it is important to target misfolded glycoproteins for degradation.,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the glycosyl hydrolase 47 family.,tissue specificity:Widely expressed.,

• Subcellular Location：
• Endoplasmic reticulum membrane ; Single-pass type II membrane protein .

• Expression：
• Widely expressed.

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