• Target：
• LPAAT-θ

• Fields：
• >>Glycerolipid metabolism;>>Glycerophospholipid metabolism;>>Metabolic pathways

• Gene Name：
• AGPAT9

• Protein Name：
• Glycerol-3-phosphate acyltransferase 3

• Human Gene Id：
• 84803

• Human Swiss Prot No：
• Q53EU6

• Mouse Gene Id：
• 231510

• Mouse Swiss Prot No：
• Q8C0N2

• Rat Gene Id：
• 305166

• Rat Swiss Prot No：
• Q4V8J4

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human AGPAT9. AA range:381-430

• Specificity：
• LPAAT-θ Polyclonal Antibody detects endogenous levels of LPAAT-θ protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• AGPAT9;GPAT3;MAG1;HMFN0839;Glycerol-3-phosphate acyltransferase 3;GPAT-3;1-acylglycerol-3-phosphate O-acyltransferase 9;1-AGP acyltransferase 9;1-AGPAT 9;Acyl-CoA:glycerol-3-phosphate acyltransferase 3;hGPAT3;Lung cancer metastas

• Observed Band(KD)：
• 48kD

• Background：
• This gene encodes a member of the lysophosphatidic acid acyltransferase protein family. The encoded protein is an enzyme which catalyzes the conversion of glycerol-3-phosphate to lysophosphatidic acid in the synthesis of triacylglycerol. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2012],

• Function：
• catalytic activity:Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.,domain:The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.,enzyme regulation:Inhibited by N-ethylmaleimide (NEM).,function:Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Overexpression activates the mTOR pathway.,pathway:Glycerolipid metabolism; triacylglycerol biosynthesis.,pathway:Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.,similarity:Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.,tissue specificity:Widely expressed. Expressed in liver, kidney, testis, brain, heart, skeletal muscle, thyroid, prostate, thymus and placenta.

• Subcellular Location：
• Endoplasmic reticulum membrane ; Multi-pass membrane protein .

• Expression：
• Widely expressed. Expressed in liver, kidney, testis, brain, heart, skeletal muscle, thyroid, prostate, thymus and placenta. Also expressed lung and adipose tissue.

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