JNK1/2/3 Polyclonal Antibody

    • Catalog No.:YT2440
    • Applications:WB,IHC-p,IF,ELISA
    • Reactivity:Human,Mouse,Rat,Chicken(testedbyourcustomer)
      • Gene Name:
      • MAPK8/9/10
      • Protein Name:
      • Mitogen-activated protein kinase 8/9/10
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human SAPK/JNK. AA range:166-215
      • Specificity:
      • JNK1/2/3 Polyclonal Antibody detects endogenous levels of JNK1/2/3 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • MAPK8; JNK1; PRKM8; SAPK1; SAPK1C; Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; MAPK9; JNK2; PRKM9; SAPK1A; Mi
      • MolecularWeight(Da):
      • 48296/48139/52585
      • Observed Band(KD):
      • 44
      • Background:
      • mitogen-activated protein kinase 8(MAPK8) Homo sapiens The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spl
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. Inhibited by dual specificity phosphatases, such as DUSP1.,function:JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.,function:Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP
      • Subcellular Location:
      • intracellular,nucleus,nucleoplasm,mitochondrion,cytosol,
      • Expression:
      • Brain,Epithelium,Fetal brain,Lung,Pooled,Testis,

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      Fan, Dong-xiao, et al. "17β-Estradiol on the Expression of G-Protein Coupled Estrogen Receptor (GPER/GPR30) Mitophagy, and the PI3K/Akt Signaling Pathway in ATDC5 Chondrocytes In Vitro." Medical science monitor: international medical journal of experimental and clinical research 24 (2018): 1936.

      Ma, Xingzhe, et al. "Tamoxifen induces the development of hernia in mice by activating MMP-2 and MMP-13 expression." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 1852.5 (2015): 1038-1048.

      • Products Images
      • Western Blot analysis of various cells using JNK1/2/3 Polyclonal Antibody diluted at 1:1000
      • Western Blot analysis of hela cells using JNK1/2/3 Polyclonal Antibody diluted at 1:1000
      • Immunofluorescence analysis of LOVO cells, using SAPK/JNK Antibody. The picture on the right is blocked with the synthesized peptide.
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SAPK/JNK Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from LOVO cells, using SAPK/JNK Antibody. The lane on the right is blocked with the synthesized peptide.