• Target：
• JNK1/2/3

• Fields：
• >>Endocrine resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>cAMP signaling pathway;>>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Mitophagy - animal;>>Autophagy - animal;>>Protein processing in endoplasmic reticulum;>>Apoptosis;>>Apoptosis - multiple species;>>Necroptosis;>>Wnt signaling pathway;>>Osteoclast differentiation;>>Focal adhesion;>>Tight junction;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>IL-17 signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>TNF signaling pathway;>>Neurotrophin signaling pathway;>>Retrograde endocannabinoid signaling;>>Dopaminergic synapse;>>Inflammatory mediator regulation of TRP channels;>>Insulin signaling pathway;>>GnRH signaling pathway;>>Progesterone-mediated oocyte maturation;>>Pr

• Gene Name：
• MAPK8/9/10

• Protein Name：
• Mitogen-activated protein kinase 8/9/10

• Human Gene Id：
• 5599/5601/5602

• Human Swiss Prot No：
• P45983/P45984/P53779

• Mouse Gene Id：
• 26419/26420

• Rat Gene Id：
• 116554/50658/25272

• Rat Swiss Prot No：
• P49185/P49186/P49187

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human SAPK/JNK. AA range:166-215

• Specificity：
• JNK1/2/3 Polyclonal Antibody detects endogenous levels of JNK1/2/3 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MAPK8;JNK1;PRKM8;SAPK1;SAPK1C;Mitogen-activated protein kinase 8;MAP kinase 8;MAPK 8;JNK-46;Stress-activated protein kinase 1c;SAPK1c;Stress-activated protein kinase JNK1;c-Jun N-terminal kinase 1;MAPK9;JNK2;PRKM9;SAPK1A;Mi

• Observed Band(KD)：
• 46kD,54kD

• Background：
• The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spl

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. Inhibited by dual specificity phosphatases, such as DUSP1.,function:JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.,function:Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP

• Subcellular Location：
• Cytoplasm . Nucleus . Cell junction, synapse . In the cortical neurons, predominantly cytoplasmic and associated with the Golgi apparatus and endosomal fraction. Increased neuronal activity increases phosphorylated form at synapses (By similarity). Colocalizes with POU5F1 in the nucleus. .

• Expression：
• Brain,Epithelium,Fetal brain,Lung,Pooled,Testis,

NCX1 disturbs calcium homeostasis and promotes RANKL-induced osteoclast differentiation by regulating JNK/c-Fos/NFATc1 signaling pathway in multiple myeloma

IL-17A promotes endothelial cell senescence by up-regulating the expression of FTO through activating JNK signal pathway BIOGERONTOLOGY Li, Na, Luo, Runan, Zhang, Wenlong, Wu, Yu, Hu, Chaojie, Liu, Manli, Jiang, Diya, Jiang, Ziran, Zhao, Xinxin, Wang, Yiping, Li, Qing WB Human HUVECs

Proliferatins suppress lipopolysaccharide-induced inflammation via inhibition of the NF-κB and MAPK signaling pathways. BIOORGANIC CHEMISTRY2022 Jul;124:105810. Mouse RAW264.7 macrophages

Therapeutic Potential of Synthetic Human β-Defensin 1 Short Motif Pep-B on Lipopolysaccharide-Stimulated Human Dental Pulp Stem Cells Mediat Inflamm. 2022;2022:6141967. WB Human 1 : 1000

Selected lactobacilli strains inhibit inflammation in LPS-induced RAW264.7 macrophages by suppressing the TLR4-mediated NF-κB and MAPKs activation Food Sci Tech-Brazil. 2022 Mar;42:. WB Mouse 1:500

17β-Estradiol on the Expression of G-Protein Coupled Estrogen Receptor (GPER/GPR30) Mitophagy, and the PI3K/Akt Signaling Pathway in ATDC5 Chondrocytes In Vitro. MEDICAL SCIENCE MONITOR Med Sci Monitor. 2018; 24: 1936–1947 WB Mouse ATDC5 cell

Novel paeonol derivatives: Design, synthesis and anti-inflammatory activity in vitro and in vivo. BIOORGANIC CHEMISTRY Bioorg Chem. 2020 May;98:103735 WB Mouse RAW264.7 cell

Astragalus Polysaccharide Inhibits Ionizing Radiation-Induced Bystander Effects by Regulating MAPK/NF-κB Signaling Pathway in Bone Mesenchymal Stem Cells (BMSCs). MEDICAL SCIENCE MONITOR Med Sci Monitor. 2018; 24: 4649–4658 WB Human BMSCs

Effect of cold stress on the MAPK pathway and lipidomics on muscle of Takifugu fasciatus. AQUACULTURE Aquaculture. 2021 Jul;540:736691 WB Fish 1:1500 Muscles

Soybean Glycinin- and β-Conglycinin-Induced Intestinal Damage in Piglets via the p38/JNK/NF-κB Signaling Pathway. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY J Agr Food Chem. 2018;66(36):9534–9541 WB,IHC Pig 1:500,1:1000 small intestine

Astragalus Polysaccharide Eases G1 Phase-Correlative Bystander Effects through Mediation of TGF-βR/MAPK/ROS Signal Pathway After Carbon Ion Irradiation in BMSCs. AMERICAN JOURNAL OF CHINESE MEDICINE Am J Chinese Med. 2019 May;47(03):595-612 WB Human BMSCs

Inhibitory effects of oxymatrine on TGF‑β1‑induced proliferation and abnormal differentiation in rat cardiac fibroblasts via the p38MAPK and ERK1/2 signaling pathways. Molecular Medicine Reports Mol Med Rep. 2017 Oct;16(4):5354-5362 WB Rat 1:1000 cardiac fibroblast (CFB)

Dibromoacetic Acid Induced Hepatotoxicity in Mice through Oxidative Stress and Toll-Like Receptor 4 Signaling Pathway Activation. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2019;2019:5637235 WB Mouse Liver

Administration of SB203580, a p38 MAPK Inhibitor, Reduced the Expression of MMP9, and Relieved Neurologic Severity in the Experimental Autoimmune Neuritis (EAN) in Rats. NEUROCHEMICAL RESEARCH Neurochem Res. 2015 Jul;40(7):1410-1420 WB Rat 1:1000 sciatic nerves

Adipose-Derived Stem Cells Protect Ischemia-Reperfusion and Partial Hepatectomy by Attenuating Endoplasmic Reticulum Stress. Frontiers in Cell and Developmental Biology Front Cell Dev Biol. 2020 Mar;0:177 WB Pig Liver

Tamoxifen induces the development of hernia in mice by activating MMP-2 and MMP-13 expression. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE 2015 Feb 19 WB Mouse NIH3T3 cell

Cadmium induces BNIP3-dependent autophagy in chicken spleen by modulating miR-33-AMPK axis. CHEMOSPHERE 2017 Dec 06 WB Chicken 1:500 Spleen

The Effects of Maternal Atrazine Exposure and Swimming Training on Spatial Learning Memory and Hippocampal Morphology in Offspring Male Rats via PSD95/NR2B Signaling Pathway. CELLULAR AND MOLECULAR NEUROBIOLOGY Cell Mol Neurobiol. 2019 Oct;39(7):1003-1015 WB Rat 1:1000 hippocampus

Molecular Mechanisms of IRE1α-ASK1 Pathway Reactions to Unfolded Protein Response in DRN Neurons of Post-Traumatic Stress Disorder Rats. JOURNAL OF MOLECULAR NEUROSCIENCE J Mol Neurosci. 2017 Apr;61(4):531-541 WB Rat 1:500 brain

Telekin suppresses human hepatocellular carcinoma cells in vitro by inducing G 2 /M phase arrest via the p38 MAPK signaling pathway. ACTA PHARMACOLOGICA SINICA Acta Pharmacol Sin. 2014 Sep;35(10):1311-1322 WB Human HepG2 cell

Lycium barbarum Polysaccharides Protect Rat Corneal Epithelial Cells against Ultraviolet B-Induced Apoptosis by Attenuating the Mitochondrial Pathway and Inhibiting JNK Phosphorylation. Biomed Research International Biomed Res Int. 2017;2017:5806832 WB Rat 1 : 250 RCE cell

NOV inhibits proliferation while promoting apoptosis and migration in osteosarcoma cell lines through p38/MAPK and JNK/MAPK pathways. ONCOLOGY REPORTS 2015 Jul 24 WB Human 143B cell

Astragalus Polysaccharide Inhibits Ionizing Radiation-Induced Bystander Effects by Regulating MAPK/NF-κB Signaling Pathway in Bone Mesenchymal Stem Cells (BMSCs). MEDICAL SCIENCE MONITOR Med Sci Monitor. 2018; 24: 4649–4658 WB Human BMSCs

Interfering RNA against PKC-α inhibits TNF-α-induced IP3R1 expression and improves glomerular filtration rate in rats with fulminant hepatic failure. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY Am J Physiol-Renal. 2018 May;314(5):F942-F955 WB Rat Glomerular mesangial cells (GMCs)

Protective effects of flavonoids from the leaves of Carya cathayensis Sarg. against H₂O₂‑induced oxidative damage and apoptosis in vitro. Experimental and Therapeutic Medicine Exp Ther Med. 2021 Dec;22(6):1-11 WB Rat 1:1000 Rat aortic endothelial cells (RAECs)

Ping-Chong-Jiang-Ni Formula Induces Apoptosis and Inhibits Proliferation of Human Ectopic Endometrial Stromal Cells in Endometriosis via the Activation of JNK Signaling Pathway. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2017;2017:6489427 WB Human 1 : 1000 EESCs

Cadmium induces BNIP3-dependent autophagy in chicken spleen by modulating miR-33-AMPK axis. CHEMOSPHERE 2017 Dec 06 WB Chicken 1:1000 Spleen

Targeting androgen receptor with ASC-J9 attenuates cardiac injury and dysfunction in experimental autoimmune myocarditis by reducing M1-like macrophage. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Biochem Bioph Res Co. 2017 Apr;485:746 WB Mouse 1:500 Raw264.7 macrophages

Alpha-Momorcharin Inhibits Proinflammatory Cytokine Expression by M1 Macrophages but Not Anti-Inflammatory Cytokine Expression by M2 Macrophages Journal of Inflammation Research Fubing Shen WB Human

Ershiwuwei Lvxue Pill alleviates rheumatoid arthritis by different pathways and produces changes in the gut microbiota PHYTOMEDICINE Shaohui Wang WB Rat

Hypoxic preconditioned mesenchymal stem cells ameliorate rat brain injury after cardiopulmonary resuscitation by suppressing neuronal pyroptosis. Feng Chen WB Rat cortical tissue

2-phenylacetamide Separated from the seed of Lepidium apetalum Willd. inhibited renal fibrosis via MAPK pathway mediated RAAS and oxidative stress in SHR Rats. Wei-sheng Feng WB Rat renal tissue

Protective effect of cornuside on OGD/R injury in SH-SY5Y cells and its underlying mechanism. BRAIN RESEARCH Jianwei Gong WB Human SH-SY5Y cell

IRE1α-JNK axis activation contributes to intracellular lipid accumulation in calf hepatocytes JOURNAL OF DAIRY SCIENCE Wenwen Gao WB Calf 1:1000 hepatocytes

Design and synthesis of pterostilbene derivatives bearing triazole moiety that might treat DSS-induced colitis in mice through modulation of NF-κB/MAPK signaling pathways EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY Liuzeng Chen WB Mouse 1:1000 RAW264.7 cell

Design, synthesis, in vitro and in vivo biological evaluation of pterostilbene derivatives for anti-inflammation therapy JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY Liuzeng Chen WB Mouse 1:1000 RAW264.7 cell

PANX1-mediated ATP release confers FAM3A’s suppression effects on hepatic gluconeogenesis and lipogenesis Military Medical Research Hu Cheng-Qing WB Mouse hepatocytes

Silencing miR-155-5p Alleviates Hippocampal Damage in Kainic Acid-Induced Epileptic Rats via the Dusp14/MAPK Pathway BRAIN RESEARCH BULLETIN Qiong Fang WB,IHC Rat 1:300,1:1000 hippocampal tissue

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs