• Target：
• IκB-α

• Fields：
• >>cAMP signaling pathway;>>Chemokine signaling pathway;>>NF-kappa B signaling pathway;>>Apoptosis;>>Osteoclast differentiation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>Cytosolic DNA-sensing pathway;>>C-type lectin receptor signaling pathway;>>IL-17 signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>TNF signaling pathway;>>Neurotrophin signaling pathway;>>Adipocytokine signaling pathway;>>Relaxin signaling pathway;>>Insulin resistance;>>Alcoholic liver disease;>>Epithelial cell signaling in Helicobacter pylori infection;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Legionellosis;>>Yersinia infection;>>Leishmaniasis;>>Chagas disease;>>Toxoplasmosis;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Human cytomegalovirus infection;>>Influenza A;>>Human T-cell leukemia virus 1 infection;>>

• Gene Name：
• NFKBIA IKBA MAD3 NFKBI

• Protein Name：
• NF-kappa-B inhibitor alpha

• Human Gene Id：
• 4792

• Human Swiss Prot No：
• P25963

• Mouse Gene Id：
• 18035

• Mouse Swiss Prot No：
• Q9Z1E3

• Rat Gene Id：
• 25493

• Rat Swiss Prot No：
• Q63746

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human IkappaB-alpha. AA range:15-64

• Specificity：
• IκB-α Polyclonal Antibody detects endogenous levels of IκB-α protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NFKBIA;IKBA;MAD3;NFKBI;NF-kappa-B inhibitor alpha;I-kappa-B-alpha;IkB-alpha;IkappaBalpha;Major histocompatibility complex enhancer-binding protein MAD3

• Observed Band(KD)：
• about 40kd

• Background：
• This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011],

• Function：
• disease:Defects in NFKBIA are the cause of ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant (ADEDAID) [MIM:612132]. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ADEDAID is an ectodermal dysplasia associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection.,function:Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.,induction:Induced in adherent monocytes.,online information:NFKBIA mutation

• Subcellular Location：
• Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. .

• Expression：
• Brain,Kidney,Lymph node,Monocyte,

Radiation-induced bystander effect on the genome of bone marrow mesenchymal stem cells in lung cancer

Tyrosol inhibits NF-κB pathway in the treatment of enterotoxigenic Escherichia coli-induced diarrhea in mice MICROBIAL PATHOGENESIS Fazheng Yu, Jian Guo, Hong lin Ren, Shiying Lu, Zhaoqi He, Jiang Chang, Xueyu Hu, Ruoran Shi, Yuanyuan Jin, Yansong Li, Zengshan Liu, Xiaoxu Wang, Pan Hu WB Mouse colonic tissue

Zhongfeng Capsules protects against cerebral ischemia–reperfusion injury via mediating the phosphoinositide 3-kinase/Akt and toll-like receptor 4/nuclear factor kappa B signaling pathways by regulating neuronal apoptosis and inflammation WB Rat 1:1000 brain tissue/

PRKAR2A-derived circular RNAs promote the malignant transformation of colitis and distinguish patients with colitis-associated colorectal cancer Clin Transl Med. 2022 Feb;12(2):e683. WB Mouse 1:1000 colon

Electroacupuncture Prevents the Depression-Like Behavior by Inhibiting the NF-κB/NLRP3 Inflammatory Pathway in Hippocampus of Mice Subjected to Chronic Mild Stress Neuropsychobiology. ;:1-9. WB Mouse 1:1000 hippocampus

Berberine hydrochloride attenuates lipopolysaccharide-induced endometritis in mice by suppressing activation of NF-κB signal pathway. INTERNATIONAL IMMUNOPHARMACOLOGY 2014 Nov 15 WB Mouse uteri

MAST3 modulates the inflammatory response and proliferation of fibroblast-like synoviocytes in rheumatoid arthritis. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2019 Dec;77:105900 WB Rat 1:1000 Synovial tissues FLSs

NLRC5 promotes cell proliferation via regulating the NF-κB signaling pathway in Rheumatoid arthritis. MOLECULAR IMMUNOLOGY Mol Immunol. 2017 Nov;91:24 WB Rat 1:500 Synovial tissues FLS cell

Renin-angiotensin system activation accelerates atherosclerosis in experimental renal failure by promoting endoplasmic reticulum stress-related inflammation. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Int J Mol Med. 2017 Mar;39(3):613-621 WB Mouse 1:500 aorta tissue RAW264.7 macrophages

PSTPIP2 Inhibits the Inflammatory Response and Proliferation of Fibroblast-Like Synoviocytes in vitro. Frontiers in Pharmacology Front Pharmacol. 2018 Dec;9:1432 WB Rat 1:1000 Synovial tissues Fibroblast-like synoviocytes (FLSs)

Overexpression of juxtaposed with another zinc finger gene 1 reduces proinflammatory cytokine release via inhibition of stress‐activated protein kinases and nuclear factor‐κB. FEBS Journal 2014 Jun 17 WB Mouse Hepatic tissues

Lactoferrin suppresses lipopolysaccharide-induced endometritis in mice via down-regulation of the NF-κB pathway. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2015 Sep;28:695 WB Mouse 1:1000 uterine tissues

The antitumor effect of hinesol, extract from Atractylodes lancea (Thunb.) DC. by proliferation, inhibition, and apoptosis induction via MEK/ERK and NF‐κB pathway in non–small cell lung cancer cell lines A549 and NCI‐H1299. JOURNAL OF CELLULAR BIOCHEMISTRY 2019 Jul 24 WB Human A549 cell

Anemonin Attenuates RANKL-Induced Osteoclastogenesis and Ameliorates LPS-Induced Inflammatory Bone Loss in Mice via Modulation of NFATc1. Frontiers in Pharmacology 2020 Feb 10 WB Mouse BMMs

Dopamine receptor D2 inhibition alleviates diabetic hepatic stellate cells fibrosis by regulating the TGF‐β1/Smads and NFκB pathways. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY Clin Exp Pharmacol P. 2021 Mar;48(3):370-380 WB Rat 1 : 1000 Liver hepatic stellate cells (HSCs)

Zhang, Hua, et al. "Subcutaneous administration of infliximab-attenuated silica-induced lung fibrosis." International journal of occupational medicine and environmental health 31.4 (2018): 503-515.

Liao, Jiamei, et al. "Anti-inflammatory Activity of Essential Oil from Leaves of Blumea balsamifera (L.) DC through Inhibiting TLR4/NF-kB Signaling Pathways and NLRP3 Inflammasome Activation in LPS-induced RAW264. 7 Macrophage Cells." Journal of Essential Oil Bearing Plants (2021): 1-17.

The adhesion protein of Mycoplasma genitalium inhibits urethral epithelial cell apoptosis through CypA-CD147 activating PI3K/ Akt/NF-κB pathway APPLIED MICROBIOLOGY AND BIOTECHNOLOGY Yanhua Zeng WB Human

Surface functionalization-dependent inflammatory potential of polystyrene nanoplastics through the activation of MAPK/ NF-κB signaling pathways in macrophage Raw 264.7 ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Jian Xu WB Mouse RAW 264.7 cells

Effects of Aire on perforin expression in BMDCs via TLR7/8 and its therapeutic effect on type 1 diabetes INTERNATIONAL IMMUNOPHARMACOLOGY Wei Yang WB Mouse bone marrow-derived dendritic cells (BMDCs)

Oridonin Attenuates Thioacetamide-Induced Osteoclastogenesis Through MAPK/NF-κB Pathway and Thioacetamide-Inhibited Osteoblastogenesis Through BMP-2/RUNX2 Pathway CALCIFIED TISSUE INTERNATIONAL Xu Jian WB Mouse,Rat RAW264.7 cell,bone mesenchymal stem cells (BMSCs)

Botulinum toxin type A inhibits M1 macrophage polarization by deactivation of JAK2/STAT1 and IκB/NFκB pathway and contributes to scar alleviation in aseptic skin wound healing BIOMEDICINE & PHARMACOTHERAPY Yan-ping Zou WB Rat,Mouse 1:1000 scar granular tissue Raw 264.7 cell

Buddleoside-rich Chrysanthemum indicum L. extract modulates macrophage-mediated inflammation to prevent metabolic syndrome induced by unhealthy diet BMC Complementary Medicine and Therapies Zhou Yiqing WB Rat liver tissue

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