• Target：
• IRF-3

• Fields：
• >>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>Cytosolic DNA-sensing pathway;>>Alcoholic liver disease;>>Shigellosis;>>Pertussis;>>Yersinia infection;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Human cytomegalovirus infection;>>Influenza A;>>Human papillomavirus infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Coronavirus disease - COVID-19;>>Viral carcinogenesis;>>Lipid and atherosclerosis

• Gene Name：
• IRF3

• Protein Name：
• Interferon regulatory factor 3

• Human Gene Id：
• 3661

• Human Swiss Prot No：
• Q14653

• Mouse Gene Id：
• 54131

• Mouse Swiss Prot No：
• P70671

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human IRF3. AA range:362-411

• Specificity：
• IRF-3 Polyclonal Antibody detects endogenous levels of IRF-3 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• IRF3;Interferon regulatory factor 3;IRF-3

• Observed Band(KD)：
• 48-55kd

• Background：
• This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011],

• Function：
• function:Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.,PTM:Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphoryla

• Subcellular Location：
• Cytoplasm . Nucleus . Mitochondrion . Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect (PubMed:10805757). When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm (PubMed:10805757). Recruited to mitochondria via TOMM70:HSP90AA1 upon Sendai virus infection (PubMed:25609812). .

• Expression：
• Expressed constitutively in a variety of tissues.

IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection. Nature Microbiology Nat Microbiol. 2021 May;6(7):932-945 WB Human A549 cell

Induction of PGRN by influenza virus inhibits the antiviral immune responses through downregulation of type I interferons signaling. PLoS Pathogens Plos Pathog. 2019 Oct;15(10):e1008062 WB Human HEK293 cell

Influenza virus NS1 interacts with 14-3-3ε to antagonize the production of RIG-I-mediated type I interferons VIROLOGY Jinhua Liu WB Human

Helicobacter pylori promotes gastric intestinal metaplasia through activation of IRF3-mediated kynurenine pathway. Wanfu Xu WB Human 1:1000 BGC823 cell

Targeting the mechanism of IRF3 in sepsis-associated acute kidney injury via the Hippo pathway. Xingui Dai WB Human HK-2 cell

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