IKKα/β Polyclonal Antibody

    • Catalog No.:YT2302
    • Applications:WB,IHC-p,IF(paraffin section),ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • CHUK/IKBKB
      • Protein Name:
      • Inhibitor of nuclear factor kappa-B kinase subunit alpha
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human IKK-alpha/beta. AA range:141-190
      • Specificity:
      • IKKα/β Polyclonal Antibody detects endogenous levels of IKKα/β protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B
      • MolecularWeight(Da):
      • 84654/86564
      • Observed Band(KD):
      • 85
      • Background:
      • conserved helix-loop-helix ubiquitous kinase(CHUK) Homo sapiens This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].,enzyme regulation:Activated when phosphorylated and inactivated when dephosphorylated.,function:Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.,PTM:Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK
      • Subcellular Location:
      • intracellular,nucleoplasm,cytoplasm,cytosol,IkappaB kinase complex,cytoplasmic side of plasma membrane,CD40 receptor complex,intracellular membrane-bounded organelle,
      • Expression:
      • Brain,Cervix carcinoma,Heart,Lymph,T-cell,
      • Products Images
      • Western Blot analysis of various cells using IKKα/β Polyclonal Antibody
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using IKK-alpha/beta Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HepG2 cells, using IKK-alpha/beta Antibody. The lane on the right is blocked with the synthesized peptide.